The diagnosis of CGD is typically made by NBT test
by identifying the neutrophil oxidative burst activity.
To study the serum alpha-1 antitrypsin levels in patients of CRF on long-term dialysis at different intervals (before, immediately after, and 8 hours after dialysis) and to appreciate and establish the extent of neutrophil activation in CRF patients by the Nitroblue Tetrazolium Test (NBT test).
The principle of NBT test is detection of increased enzymatic activity of neutrophils, which is capable of reducing nitroblue tetrazolium to formazan, which forms a black precipitate.
The NBT test was done on 25 patients in both pre- and postdialysis samples.
In the NBT test, the blue dye formazan is produced by the reduction of the NBT dye, a reaction dependent upon neutrophil NADPH oxidase (2).
The NBT test involves the microscopic assessment of the ability of patient neutrophils to reduce the yellow soluble redox dye NBT to form blue-black formazan, an insoluble material that precipitates intracellularly.
Complement was used as opsonin for the NBT test and was provided using fresh pooled human serum.
After engineers have selected as many instruments as they need to implement their NBT test
strategy, ScanWorks FCT automatically configures, inserts, operates, and subsequently removes the FPGA-based board tester.
The NBT test (14) measures the percentage of actively phagocytosing (positively stained) neutrophils in blood smears.
A blood sample was used for the NBT test (14) in which 400 neutrophils from a blood smear were observed under 400x magnification to determine the proportion that were positively stained and showed evidence of active involvement in phagocytosis.
* Nitroblue terazolium dye (NBT) reduction test: The NBT test
was performed according to the method of Park et al.
For example, an NBT test
strategy might include boundary scan test, PCT, and a BIST technique such as IBIST.