The principle of NBT test is detection of increased enzymatic activity of neutrophils, which is capable of reducing nitroblue tetrazolium to formazan, which forms a black precipitate.
The NBT test was done on 25 patients in both pre- and postdialysis samples.
The results of the NBT tests also showed an overall increase in the NBT positivity, irrespective of the time of sampling, pre- or postdialysis.
The NBT test is dependent upon the microscopic evaluation of stained neutrophils for the presence of intracellular formazan.
The NBT test involves the microscopic assessment of the ability of patient neutrophils to reduce the yellow soluble redox dye NBT to form blue-black formazan, an insoluble material that precipitates intracellularly.
Complement was used as opsonin for the NBT test and was provided using fresh pooled human serum.
The activity of neutrophils in the NBT test depends upon the ability of the cells to both adhere to glass and to produce oxidative radicals.
After engineers have selected as many instruments as they need to implement their NBT test
strategy, ScanWorks FCT automatically configures, inserts, operates, and subsequently removes the FPGA-based board tester.
The NBT test (14) measures the percentage of actively phagocytosing (positively stained) neutrophils in blood smears.
A blood sample was used for the NBT test (14) in which 400 neutrophils from a blood smear were observed under 400x magnification to determine the proportion that were positively stained and showed evidence of active involvement in phagocytosis.
This study investigated 3 aspects of plague in its natural hosts in one of the world's major plague foci: the effect of infection on host survival, the dynamics of the antibody response to infection, and the specificity of the NBT test, which has been used as a measure of rodent infection status in previous studies.
For example, an NBT test
strategy might include boundary scan test, PCT, and a BIST technique such as IBIST.