A serum sample was tested for anti-calcium sensing receptor (CaSR) antibodies  and NALP5
antibodies  to rule out autoimmune hypoparathyroidism and it was found to be negative; the CaSR antibody index was 1.09 (normal range: 0.57-1.38; upper limit of normal, 1.73) and the NALP5
antibody index was 1.12 (normal range: 0.62-1.93; upper limit of normal, 2.17).
In addition, studies from our group showed that AIRE significantly up-regulated the mRNA expression levels of Ins2, glutamate decarboxylase 65/67 (GAD65/67), insulinoma-associated protein 2 (IA-2), IGRP, retinol-binding protein 3 (Rbp3), salivary protein 1 (Spt1), pyrin domain containing 5 (Nalp5
), and mylin-oligodendrocyte glycoprotein (Mog) in AIRE-overexpressing bone marrow-derived dendritic cells (BMDCs), DC2.4 cells, and RAW264.7 cells.