In addition to up regulating the expression levels of antioxidant enzymes, PPARI3 downregulates NADPH
oxidase was measured based on its capacity to reduce ferricytochrome c in ferrocytochrome at pH of 7.8 (22).
Leptin induces oxidative stress through activation of NADPH
oxidase in renal tubular cells: Antioxidant effect of L-carnitine.
The NADP+ and NADPH
levels were quantified via enzymatic cycling, using the phenazine methosulfate (PMS)-catalyzed reduction thiazolyl blue (MTT) method in the presence of glucose 6-phosphate (G6P) for NAD[P.sup.+], and glucose 6-phosphate dehydrogenase (G6PDH) for NADPH
After hypoxia/reoxygenation (H/R) treatment for 24 h, (A) intracellular ROS production was analyzed by H2DCF-DA in H9c2 cells; (B) mitochondria ROS production was detected by MitoSOX Red assay; (C) the activity of NADPH
oxidase was measured by chemiluminescence assay; (D) the protein level of NOX2 was examined by western blot.
Km and Vmax values for NADPH
were 85 [mirco]mol and 4.8 [mirco]mol/min/mg, respectively (Figure 5).
Moreover, OS was prominent in the juvenile female mouse model of NAFLD/NASH and the mechanism might be related to the activation of the liver NADPH
Inhibition of diaphorase activity of PfFNR was measured in the reaction of Scheme 1 as described above, by the additions of each alkaloid at 100 ppm, NADPH
at 100 uM, and 5 nM FNR.
Caption: FIGURE 1: Extract ion (m/z 956 [right arrow] 817) chromatograms obtained from LC-LTQ MS analysis of microsomal incubations containing LIM, GSH, NAL, and NADPH
in the absence microsomes (a), or in presence of HLMs (b) or MLMs (c).
Oxidase Pathway Mediates hBM-MSC Inhibition of HSC Activation.
Accordingly, although high glucose levels did not change EMPs' morphology (i.e., size and antigen composition), the hyperglycemia increases the functionality of EMPs in activating NADPH
oxidase/[p38.sup.phox]-mediated signaling when compared to EMPs formed in a normoglycemic environment .