Keywords: Aphanius fasciatus, genetic differentiation, NADH-dehydrogenase subunit1, Tunisia
The NADH-dehydrogenase subunit 1 was a valuable molecular marker for evolutionary relationship reconstructions among populations of fish species (Palumbi, 1996).
NADH1 gene was amplified from total DNA extracts using newly defined primers, respectively designed on the basis of the NADH-dehydrogenase subunit 1(ND1) of Aphanius fasciatus (GenBank Accession no.
The published sequence of NADH-dehydrogenase subunit 1(ND1) of A.
Rotenone is a naturally occurring insecticide and is a well-characterized, high-affinity specific inhibitor of complex I (NADH-dehydrogenase
The ND3/ND4 region begins in the glycyl tRNA gene and spans the NADH-dehydrogenase
subunit-3, arginyl tRNA, NADH-dehydrogenase
subunit-4L, and NADH-dehydrogenase
subunit-4 genes, ending in the histidyl tRNA gene.
A mutation in the NADH-dehydrogenase
subunit 2 fibroblast aging.
The uncoupling mechanisms that may be linked to the high resistance of some species to reoxygenation-derived oxidative stress by reducing ROS production include: 1) proton sinks that involve the UCP, the bacterial mitochondrial unspecific channels, or the PT pore (PTP) of mammals; and 2) the non-pumping alternative redox enzymes including a NADH-dehydrogenase
type 2 (NDH2), the mitochondrial glycerol-3-phosphate-dehydrogenase (mtGPDH), and the AOX, each as an additional participant in a branched respiratory chain (Kadenbach 2003, Lesser 2006, Guerrero-Castillo et al.
Assuming, for heuristic purposes, an evolutionary rate of ND5 between 0.75 and 1.00% per lineage per million years based on estimates for cytochrome b in cyprinids (Dowling et al., 2002) and the observation (Meyer, 1994) that NADH-dehydrogenase
subunit genes evolve faster than other mitochondrial protein-coding genes, the two mtDNA clades likely have been evolving independently for over 7 million years.
As the electrons produced by other NADH-dehydrogenases
are not accepted by decylubiquinone (3), reduction of DCIP is almost completely caused by complex I activity, resulting in very high rotenone-sensitive activity.