SCN1A

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SCN1A

A gene on chromosome 2q24.3 that encodes a large alpha subunit of a transmembrane glycoprotein complex, which forms a voltage-gated sodium channel. The SCN1A protein product is expressed in muscle and brain.

Molecular pathology
SCN1A mutations are linked to various seizure and migraine disorders.
References in periodicals archive ?
All experiments, unless otherwise specified, were performed in 10 [K.sup.+] artificial seawater (ASW; composition in millimoles: 430 NaC1, 10 KCl, 50 Mg[C1.sub.2], 10 Ca[C1.sub.2], 10 HEPES, pH 7.5, 970 mOsm).
For this, the clots formed in vitro were washed exhaustively with high-salt buffers (0.5 M NaC1, 10 mM [CaCl.sub.2], 10 mM Tris, pH 7.3) and detergent (0.5% Triton-X 100), and were then scraped from the petri dish and transferred to microcentrifuge tubes, where they were compressed by centrifugation, washed further, and finally eluted by removal of [Ca.sup.+2] with 0.1 M EDTA.
DNA-PK holoenzyme was immunoprecipitated with PAS beads that had been coated with the Ku antibody as follows: PAS beads were pre-swollen in kinase assay buffer (50 mM HEPES, pH 7.4; 10 mM EGTA, 40 mM NaC1, 100 mM potassium acetate, 8.5 mM [CaCl.sub.2], 2.29 mM [MgCl.sub.2], 277 mM glycerol).
Binding at atmospheric pressure was determined in a volume of 100 [micro]1 The assay mixture contained 50 mM Tris HCl, pH 7.4 at the assay temperature of 5[degrees]C, 1 mM EDTA, 5 mM magnesium acetate, 10 [micro]M GDP, 1 mM dithiothreitol, 100 mM NaC1, 5 mg bovine serum albumin per milliliter, 2.5 units (at 25[degrees]C) of adenosine deaminase per milliliter, and approximately 100,000 disintegrations per min (dpm) [[S.sup.35]]GTP[S] (0.3 to 0.5 nM).
chlorotica was homogenized in an ice-cold buffer (450 mM NaC1, 1.0 mM EDTA, 5.0 mM 3-[N-morpholino]propane-sulfonic acid (MOPS), 2.3 [micro]M leupeptin, 1.0 mM dithiothreitol (DTT), 500 [micro]M phenylmethylsulfonyl fluoride (PMSF), pH 7.5) containing the mucolytic agent n-acetyl cysteine (500 mM), which is necessary to disperse the copious mucus produced by the slug (26).
To obtain final [Na.sup.+] concentrations of 200-400 mM, the 1/2 ASWs were supplemented with [Na.sup.+] in 50-mM increments using a stock solution of 4.4 M NaC1. Fertilization assays in these media were conducted as described above.
Also, rhizobial infection changed the levels of miR160, miR393, miR164, and miR168, which target ARFs (ARF10, ARF16, andARF17), TIR1, NAC1, andAGO1, respectively.
The slides were then placed for 40 min in a freshly prepared lysis buffer (2.5 M NaC1, 100 mM EDTA, 10 mM Tris, 1% Triton X-100, and 10% DMSO, pH 10).
Chua, "MicroRNA directs mRNA cleavage of the transcription factor NAC1 to downregulate auxin signals for Arabidopsis lateral root development," Plant Cell, vol.
In 100mL Erlenmeyer titration flasks 40 mL of 0.10 M NaC1 solution was taken.