Constructs-The cDNA corresponding to residues 14-131 of rat nNOS N-terminus
was amplified and cloned into the yeast two-hybrid vectors pGAD and pGBT9 as well as in the recombinant expression vector pKLSLt.
This construct has an Xpress tag at N-terminus
and DsRed2 at C-terminus (Figure 1B).
Domain 1 is situated on the N-terminus
and has a/b topology.
6) Computer prediction and domain searches of the 936-amino acid PpPBP suggested that it consists of a plastid-targeting sequence at the N-terminus
(1--55 amino acids), and TG (205-358 amino acids) and TP (558-828 amino acids) domains (Fig.
The deduced amino acid sequence has a 21 aa signal peptide sequence in the N-terminus
, and contained a manganese superoxide dismutase domain (DVWEHAYY), four conserved amino acids responsible for binding manganese H48, H96, D180 and H184 and two N-glycosylation sites NHT and, NLS.
Manufacturers of testing that involves ion exchange and capillary zone electrophoresis claim that only these techniques actually measure glycation at the N-terminus
of the beta chain; other methods, they assert, also include other glycated hemoglobins in the reported results, and in the presence of hemoglobin variants immunoassays and affinity methods may produce misleading results.
The functional role of the N- terminal insert in Thermotoga neapolitana xylose isomerase (TNXI) has been examined using several mutants indicated that the N-terminus
might be involved in correctly folding of the protein.
On exposure to ischemic environment, structure of albumin N-terminus
is changed such that it can no longer bind to cobalt.
sequences of 390 nt encoding 130 aa of Rep protein of the following begomoviruses were taken from NCBI: Cotton leaf curl Burewala virus (CLCuBuV: FR819707; AJ242974], Cotton leaf curl Kokhran virus (CLCuKoVNC_004583.
The target biomarker, hep, is a 25-residue peptide (hep25) present in urine and plasma, other N-terminus
truncated isoforms such as hep20 and hep22 were also found in humans.
To amplify the N-terminus
nonstructural region of V-105, we synthesized cDNA with primer ORF1-R14, and a DNA anchor (P-CACGAATTCACTATCGATTCTGG AACCTTCAGAGG-NH3) was linked to the N-terminus
of the cDNA by T4 RNA Ligase I (BioLabs, Tokyo, Japan).
Two repeated motifs are detected in Sa-2: (1) V*HKAAW*G (in which V* and W* can also be A and absent, respectively), present 5 times at the N-terminus
of the protein, and (2) V*HKAAGY*GGYG (in which V* and Y* can also be A and L, respectively), found 8 times in the middle and C-terminal parts of the sequence (Fig.