The ethyl acetate fraction (81 g) was subjected to column chromatography over silica gel eluting with n-hexane
, n-hexane-ethyl acetate, ethyl acetate, ethyl acetate-methanol in increasing order of polarity to get ten fractions (V1-V10).The Fraction V5 (6 g) obtained with n-hexane
: ethyl acetate (4: 6) was further chromatographed on silica gel column eluting with n-hexane
: ethyl acetate to get 3 sub-fractions VS1-VS3.
extracts: The dried extract of each sample was taken in a beaker and 100 mL of methanol was added in each, shaken well and transferred to 250 ml separating funnel.
Photographs of (a) water droplet (dyed with Rhodamine B) on top of the PE-ZIF foam, (b) N-hexane-water mixture (hexane dyed with oil-red) at the top of water, (c) immediate absorption of hexane once in contact with PE-ZIF foam, (d) the purified water and the recovered n-hexane
. [Color figure can be viewed at wileyonlinelibrary.com]
The working standard mixtures of PCDDs congeners 1-4 for spiking were separately prepared in n-hexane
from the stock standard solutions as per requirement (Table 2) to determine% recoveries of congeners.
The solvent was evaporated under reduced pressure and dissolved in water and partitioned with n-hexane
, chloroform, and ethyl acetate, in that order.
In vitro results showed that chloroform fraction at concentration of 100 ug/ml showed maximum [alpha]-amylase inhibition (53.11 +- 0.97% with IC50 value of 93.61 +- 4.44 ug/ml) followed by ethyl acetate, n-hexane
and n-butanol fractions.
Zingiber officinale Rosc, known as the medicinal plant , had moderate antibacterial activity (11.9 to 13.0 mm) against Clostridium perfringens in n-hexane
, Chloroform, ethyl acetate, and butanol extract fractions.
Crude extract was dissolved by aquadest (100 ml) and fractionated by liquid-liquid fractionation by n-hexane
and ethyl acetate (each 100 ml x 3) respectively and both evaporated in vacuum to get n-hexane
and ethyl acetate fractions.
solvent was used for the preparation of samples and ascorbic acid was used as standard in this study.
From 20 g of viscous extract, three fractions were obtained, i.e., 8.98 g of water fraction with rendement 44.9% (w/w), 0.18 g of n-hexane
fraction with rendement 0.9% (w/w), and ethyl acetate fraction as much as 5.53 g with Randement 27.65% (w/w).
All solvents (methanol, acetone, n-hexane
, and ethyl acetate) used in this study were from Wako Pure Chemical Industries, Ltd.
Organic solvents, including chloroform, acetone, ethyl acetate, and n-hexane
, were purchased from Daejung Chemical & Metals Co.