Keratin MnF, desmin, CD34, podoplanin (D2-40), S100, H-caldesmon, myogenin
, MyoD1, CD68, p63, human herpes virus 8, and factor XIII are typically negative (Figures 3 through 6).
RT-PCR analysis for myogenic markers including paired box 7 (PAX7), myogenic factor 5 (Myf5), myogenin
(MyoG), four and a half LIM domains 1 (FHL1), and nuclear factor of activated T cells 1 (NFATcl) confirmed that horse muscle cells maintained myogenic features in vitro (Figure 2C).
Markers of muscular differentiation (desmin, myogenin
, MyoD1, and smooth-muscle actin) and melanocytic markers (S100-protein, HMB-45, and melan-A) were negative.
CD99, chromogranin A, CD45, inhibin, calretinin, CA-125, ER, PR, CK7, CK20, Moc31, Tag72, myogenin
, S100, and destine were negative.
Immunohistochemical (IHC) stains Desmin and myogenin
also confirmed the rhabdomyoblastic nature of stromal cells (Figure 2A-D).
Furthermore, LPS was also shown to decrease expression of myogenic factor, including myod1 and myogenin
(MyoD1: t = 4.039, P < 0.05 and myogenin
: t = 3.300, P < 0.01), but dandelion extract was shown to against this effect of LPS (MyoD1: t = −3.160, P < 0.05 and myogenin
: t = −3.207, P < 0.01).
Expression profiles of myostatin, myogenin
, and myosin heavy chain in skeletal muscles of two rabbit breeds differing in growth rate.
Diagnosed cases of neuroblastoma on hematoxylin and eosin stain along with immunohistochemistry for round blue cell tumour panel using Synaptophysin, Neuron specific Enolase, Desmin, Myogenin
, CD45 and WT1 and referred for marrow biopsy from January 2016 to December 2017 were selected for this study.
Immunohistochemical evaluation showed no reactivity for nevomelanocytic or neural markers (S100 protein, HMB 45, Melan A, SOX10, GFAP), epithelial antigens (AE1/3) or EMA, myoid markers (desmin, SMA, myogenin
), or specific transcription factors (WT1, ALK, TFE3).
(Immunohistochemical [IHC] studies showed nonspecific staining for myoglobin and were negative for myogenin
and myo-D1.) Treatment response was moderate and primarily affected the carcinomatous component.
Immunohistochemically, they differ from other tumors by myogenic differentiation 1 (+), myogenin
(+), desmin (+) myoglobin (+), muscle specific actin (+), keratin (-) and terminal deoxynucleotidyl transferase (-) staining pattern.
Murine anti-human myogenin
, Clone: f5d, Ready for use; Monocl.