MERRF Cybrid Presents High Level of mtDNA
Mutation Load, ROS Expression and Oxidative Damage, and Reduced Mitochondrial Bioenergetics.
After interruption of anti-EGFR therapy; the KRAS
mutation load distinctly declined and remained below the limit of detection across subsequent lines of treatment.
To determine whether the cancer
mutation load is correlated with the expression levels of NEIL1, NEIL2, and NEIL3 in human cancer, mRNA expression data and somatic mutation data for 13 cancer types were obtained from the TCGA database.
The prognosis of MELAS patients depends on the location of genetic mutations,
mutation load, and the onset of disease and interventions.
We developed a method to accurately quantify the mutant load in mtDNA; using this quantitative DHPLC analysis method, we measured the levels of heteroplasmy in several DNA samples with known
mutation loads.
In such situations, the terminal
mutation load of asexual populations is equivalent to that of infinite populations, and the elimination of clones by Kondrashov's mechanism requires mutation rates greater than 1.0 per genome per generation (Howard 1994).
GABRIEL 1990
Mutation load and the survival of small populations Evolution 44:1725-1737.
This result thus demonstrated that it was possible to maintain very high lethal as well as sublethal
mutation loads and inbreeding depression, even in the face of substantial self-fertilization.
Therefore, experimental studies on epistasis between deleterious mutations have been based on indirect estimates of the individual
mutation load, by assuming a relationship between mutation number and some other experimental variable that can be known more accurately.
Following the classic Haldane principle (1937), the population genomic
mutation load only depends on the fitness mutation rate.
I also calculated and recorded average number of deleterious mutations carried in adult plants (after selection) by genotype at the special locus (AA, Aa, aa), average number of deleterious mutations by gamete type (A, a), and overall
mutation load and inbreeding depression every 20 generations during the course of each run.
(1963) have shown that the extent to which drift causes the expressed
mutation load to increase is a function of s, the selective disadvantage of a mutation.
