This suggests that the mechanisms controlling multiplicity of infection
and parasite densities follow different profiles and so are different.
The virus inoculated the Hep2 cell monolayer at a multiplicity of infection
Caption: Figure 2: Infection of human retinal endothelial cells with DENV: viral strain = Mon601; multiplicity of infection
= 1; evaluated time points postinoculation = 6, 24, 48, and 72 hours (hr).
To verify if the established EWS cell line was susceptible to VSV[DELTA]M51 infection, a confluent monolayer of these cells was inoculated with virus at a low multiplicity of infection
(MOI) of 0.01.
We isolated primary cortical neurons from C57BL/6 mice infected with 0.001 multiplicity of infection
and measured progeny particles by focus-forming assay (11).
After 2 h, the cell were infected with HSV-1 or HSV-2 at a multiplicity of infection
(MOI) of 0.001 pfu/cell, and re-incubated at 37 degC in a humidified atmosphere of 5% CO2 in air.
For the infection of primary mouse hepatocytes (PMH) by L2-MHV3, an MOI (multiplicity of infection
) of 1 was used.
Therefore, PK-15 cells were infected with equal amounts of FMDV at a multiplicity of infection
(MOI) of 0.5.
Cell monolayers were infected with a multiplicity of infection
(M.O.I.) of about 0.0004 (FluA H1N1, FluA H2N3, FluB Yamagatal, FluB Beiying, Para 3, RSV, HRV 14, CA9), and 0.008 (Adeno 5) for 1 h at 34[degrees]C.
For La Sota infection, a monolayer of DF1 cells was washed with phosphate-buffered saline (PBS) three times and 1 mL of serum-free 1640 medium containing NDV La Sota at a multiplicity of infection
(MOI) of 0.01 was then added.
After incubation for 24 hours (h), the BV-2 cells were switched to serum-free medium for 12 h and then adsorbed with JEV at a multiplicity of infection
(MOI) of 0.01 or 1 for 1h (MOI = 0.01 for the viral proliferation assay, or MOI = 1 for determining cytokines and chemokines and signaling kinase expression).
typhi strains ST8, ST8-[DELTA][pR.sub.ST98], and ST8-c-[pR.sub.ST98] were added to THP-1 macrophages at a multiplicity of infection