Columbia agar with 5% sheep blood (COS): bioMerieux (COS_bmx), Becton Dickinson (COS_BD); Trypticase Soy agar with 5% sheep blood (TSA): bioMerieux (TSA_bmx), Oxoid (TSA_Ox); Muller-Hinton agar
with 5% horse blood (MHF): bioMerieux (MHF_bmx), Oxoid (MHF_Ox); Muller-Hinton agar
with 5% sheep blood (MHB): bioMerieux (MHB_bmx); all plates used were produced by commercial manufacturers and used within their regular shelf live.
20mL of bacterial suspension was uniformly spread on the sterile petri dishes of Muller-Hinton Agar
(MHA) using sterile cotton swab and pieces of antimicrobial films were placed on the bacterial culture.
Briefly, bacterial lawns of bacterial test strains and hospital isolates were made on readymade Muller-Hinton agar
and left to dry.
jejuni isolates for antimicrobial susceptibility by disk diffusion method on Muller-Hinton agar
with 5% sheep blood and incubated them at 37[degrees]C in microaerophillic environment for 48 h before obtaining results.
A suspension of each isolate was made so that the turbidity was equal to 0.5 McFarland standard and then plated onto Muller-Hinton agar
: Suspend 38 gm of the medium in one litre of distilled water.
Antibiogram was done on Muller-Hinton agar
, and were interpreted after 24 hrs.
Agar well diffusion method is used to examine the bactericidal effect of green-synthesized silver nanoparticles using the Muller-Hinton agar
plates against the strains of Citrobacter freundii and Erwinia cacticida, which are isolated from the infected agricultural crops leaves such as Abelmoschus esculentus and Citrullus lanatus.
To isolate and identify the phenotype and genotype of [bla.sub.NDM-1]-positive bacteria, we repeatedly spread the 20 culture swab specimens onto Muller-Hinton agar
(Nissui, Tokyo, Japan) containing 100 mg/L vancomycin (Nakalai, Kyoto, Japan) plus 0.5 mg/L meropenem (LKT Laboratories, St.
The antibiotic susceptibility testing of the isolates were done on Muller-Hinton agar
using standard Kirby-Bauer disc diffusion method.
The gradient plates of Muller-Hinton agar
were prepared with different concentrations of vancomycin and linezolid.
The test was performed on Muller-Hinton agar
with 4% NaCl for oxacillin and without 4% NaCl for cefoxitin.