Vancomycin Sensitivity test was done with Enterococcus species by E Test Strip using Mueller-Hinton agar
. MIC determination paper strip which is coated with Vancomycin in a concentration gradient manner, capable of showing MICs in the range of 0.016 mcg/ml to 256 mcg/ml, on testing against the Enterococcus organism.
jejuni suspension was spotted on Mueller-Hinton agar
(negative control) or Mueller-Hinton agar
plates supplemented with 2% and 4% NaCl.
aeruginosa were processed for the determination of sensitivity against antibiotics, piperacillin, piperacillin + tazobactam, ticarcillin + tazobactam, ceftazidime, cefepime, aztreonam, imipenem, meropenem, gentamicin, amikacin, ciprofloxacin, levofloxacin, on Mueller-Hinton agar
. Quality control of the test was done by standard ATCC strain P.
Microbial cultures (cell suspension of 1 x [10.sup.8] cells/mL) were inoculated with a sterile swab onto the surface of Mueller-Hinton agar
in a Petri dish.
All strains of bacteria were grown on Mueller-Hinton Agar
In vitro antimicrobial susceptibility tests were performed on Mueller-Hinton agar
(Oxoid, UK) using Kirby-Bauer disk disc diffusion technique .
CAZ+CL and CTX+CL discs were applied on the inoculated Mueller-Hinton agar
All microbial cultures were re-isolated thrice successively on Mueller-Hinton agar
(Merck) with incubation period of 24 h at 37 degC and identity confirmed by standard bacteriological methods .
Freshly prepared cation-supplemented Mueller-Hinton agar
and Mueller-Hinton Broth (Oxoid, Thermo Fisher, British) were used for this study.
Antimicrobial susceptibilities were determined via Etest (bioMerieux) on blood Mueller-Hinton agar
(BBL), as described in the Clinical and Laboratory Standards Institute M45 document (Table 3).
Using a sterile inoculation loop, scrape spores from an agar plate and spread evenly on the surface of a Mueller-Hinton agar
Using a sterile cotton swab (Hi-Media Lab, Mumbai) the bacterial inoculums were inoculated in Mueller-Hinton agar
. The test compounds for various concentrations 5, 10, 15, 20, 25, 30, 35, and 40 [micro]g (w/v) were loaded on sterile Whatman paper disk (10 mm dia, Hi-Media Lab, Mumbai) and were placed in the center of the Mueller Hinton agar and inoculated with the test organism at 28[degrees]C.