Most of these are phenotypic methods which include Congo red assay (CRA), Air liquid interface (ALI), tissue culture plate (TCP) method and Microtiter plate
How to deal with lipophilic and volatile organic substances in microtiter plate
Results: Of 81 isolates, biofilm production was detected in 43% isolates using Congo red method while microtiter plate
assay showed biofilm production in 92% isolates.
They used microtiter plate
assays (MPAs), scanning electron microscopy (SEM) and atomic force microscopy (AFM) for their analysis.
Biofilm Production by Microtiter Plate
Because each well of an xCELLigence microtiter plate
can acquire thousands of data points, each individual well yields a complete time course for biofilm deposition or dissipation, greatly reducing the number of wells/plates needed and the total workload.
OD value of Biofilm Formation Microtiter Plate
Method Less than 0.120 Non-biofilm producer In the range of 0.120-0.240 Moderate Biofilm Producer Greater than 0.240 Strong Biofilm producer RESULTS
After being incubated in 96-well microtiter plate
for 24 h, the biofilm was gently washed with phosphate buffered saline (PBS), fixed with 95% methanol, washed with PBS, stained with 0.5% crystal violet for 30 min, and then washed with PBS.
The purified pectinase was tested for its ability to inhibit biofilm formation and adhesion by all biofilm producing bacteria(Congo red agar (CRA) and microtiter plate
Samples were placed in a microtiter plate
and bacterial growth was assessed by measuring the OD of the microtiter plate
wells at scheduled 2 h time intervals at 595 nm (Thermo Scientific, Multiskan Spectrum).
The kit provides enough material to perform 50 determinations in a 1 mL cuvette, or 192 determinations in a microtiter plate
(plus additional material for controls).
An aliquot of 0.1 mL was withdrawn from each standard and sample and placed in corresponding wells of a 96-well microtiter plate
. The absorbances were then measured at a wavelength of 562 nm using a microtiter plate