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This variability could have been caused by individual-based differences in glucuronidation activities, which also influence the performance of pooled microsomes (Kuester and Sipes 2007; Street et al.
For example, kidney microsomes exhibited a 6-fold lower [K.sub.M] value than liver ones for LCA-3G production (Figure 4(a)).
Caption: Figure 2: Metabolism profiles of alantolactone and isoalantolactone incubated with rat liver microsomes. Data are presented as mean [+ or -] SD, n = 3.
Volker Auwarter: I would slightly disagree, because marker identification can also be performed effectively by using prepared human liver microsome assays, which are less expensive and time-consuming.
Cytochrome p450-mediated metabolism of the HIV-1 protease inhibitor ritonavir (ABT-538) in human liver microsomes. J Pharmacol Exp Ther 1996; 277:423-431.
Immunoblot analysis of hepatic mitochondrial and microsome proteins from wild-type control, BNF-, and resveratrol-treated mice (n = 4each) following seven days of treatment.
Similar to what has happened in the CYP-1A2 identification, in the present study, CYP-2C19 could be identified as LM target antigen when IB was performed with human liver microsomes, although the IF was performed on rodent tissue.
The intrinsic clearance in rat and human liver microsomes (0.5 mg/mL protein) and hepatocytes (1 million cells/ mL) was conducted at 0.5 [micro]M of LDT5, diclofenac and cocktail of positive control (diclofenac, 7-hydroxycoumarin and testosterone).
In relation to Cannabis, it has been found that cannabidiol (CBD) was metabolized by CYP1A1, 1A2, 2C9, 2C19, 2D6, 3A4 and 3A5 isoenzymes in human liver microsomes, (66) and that CBD can potently inhibit the CYP3A4 and CYP3A5 isoenzymes (67) which may be clinically significant for pharmaceutical medications metabolised by these enzymes, particularly medicines of narrow therapeutic index; however, further research is required.
On stipulated day the animals were slaughtered, their livers taken out and microsomes prepared for the estimation of activities of various membrane bound DME such as cytochrome P450 (CP), cytochrome b5 (CB), cytochrome-c-reductase (CCR), aniline hydroxylase (AH), acetanilide hydroxylase (AAH), benzphetamine demethylase (BD), aminopyrine demethylase (APD), N.N.
A study describing testosterone metabolism in vitro using fresh and frozen pigskin as well as human skin microsomes was published in 2014.