Compared to standard widefield microscopy, confocal
laser scanning microscopy delivers a sharp image derived only from a defined focal plane.
Specific techniques are then treated in separate contributions, including holographic microscopy, confocal
laser scanning microscopy, optical projection tomography, electron microscopy, magnetic resonance and X-ray imaging, thin light-sheets, and ex-situ macro photography, with each chapter giving an introduction and discussing technical specifications, operator considerations, ideal environments, and tradeoffs.
7-AAD/DNA complexes can be excited by argon-ion laser and emit fluorescence with a maxima of 647 nm, making this nucleic acid stain useful for multicolor fluorescence microscopy, confocal
laser-scanning microscopy and flow cytometry.
As one of the most important advances achieved in optical microscopy, confocal
laser scanning microscopy (CLSM) offers several advantages over conventional fluorescence microscopy, including control of depth of field, elimination of image-degrading out-of-focus information, and collection of serial optical sections from thick specimens.12 However, the need for minimal tissue AF is more critical to the success of colocalization studies using CLSM than to those using standard fluorescence microscopy, because fluorescence signals are detected using colorblind photomultiplier tubes with CLSM but color-sensitive charge-coupled device cameras are used with standard fluorescence microscopy.
Unlike standard microscopy, confocal
microscopy can strongly suppress out-of-focus light.
It is one of the market leaders in each of its business areas: Microscopy, Confocal
Laser Scanning Microscopy with corresponding Imaging Systems, Specimen Preparation, and Medical Equipment.
After fluorescent microspheres were injected into the blood, samples of the digestive gland were excised, arterioles were prepared for microscopy, confocal
laser scanning micrographs were collected and microspheres and cells were counted.
One of two major forms of laser scanning microscopy, confocal
laser scanning microscopy uses a beam of laser light focused into a small point of a specimen and can be moved with a computer-controlled scanning mirror.
Investigators did so by using computer simulations of particle gelation; studying product structure using light microscopy, confocal
laser scanning microscopy, electron microscopy and dynamic light scattering of gels and emulsions; examining small-deformation rheology and large-deformation rheology of gels and emulsions; and using surface chemical and physical methods on dairy emulsions.
This technology can be applied to images of different magnifications, resolution, and modalities, including electron microscopy, optical microscopy, confocal
microscopy, radiology, magnetic resonance imaging (MRI), conventional photography, and other sophisticated imaging variants.
In contrast to standard microscopy, confocal
microscopy typically uses a laser as a light source.