of each PCR product was electrophoresed on a 1.5% agarose gel, stained with ethidium bromide and visualized with UV light.
Four wells of 5 mm in diameter were made on each plate and fifty microlitre
of each concentration (10, 20, 50 and 100 g/ml HgCl2) was added in wells of each plate separately.
" MicroMed uses microfluidics technology for precise handling of liquid sample volumes as low as microlitre
, in contrast to millilitres of sample required for analysis using traditional techniques.
About 100 microlitre
of each test bacterium was spread with the help of sterile spreader on to a sterile Mueller-Hinton agar plate, so as to achieve a confluent growth.
of the reaction mixture was then analyzed by submarine gel electrophoresis using 1.0% agarose with ethidium bromide at 8 V/ cm and the reaction product was visualized under Gel Doc/UV trans-illuminator.
Micro- capillary usually comes with graduation marks for every microlitre
that assist the observation of capillary action up to a certain volume.
vivax parasites per microlitre
of blood when compared with the gold standard (12).
After incubation, a drop of suspension (40 microlitre
) was placed on a clean glass slide and mounted with a flame-sterilised glass cover slip and examined under microscope (10x and 40x) for germ tube formation.
Subsequently 10 microlitre
of the reaction mixture was analyzed by submarine gel electrophoresis using 1.0% agarose with ethidium bromide at 8 V/ cm and the reaction product was envisioned below Gel Doc/UV trans-illuminator.
of RNAse (10mg/ml) was added to remove RNA and incubated at 37 [degrees]C for 30 min.