The use of micrococcal nuclease
digestion in DNA fragmentation may be more biologically relevant than ultrasonic shearing because plasma DNA exhibits nonrandom fragmentation patterns related to the positioning of nucleosomes (7, 8).
NETs treated with micrococcal nuclease
(MNase) showed specific DNA bands of under 100 bp in size (Figure 1(b), left panel).
A micrococcal nuclease
homologue in RNAi effector complexes.
With the discovery of newer nucleases and multifunctional enzymes like micrococcal nuclease
and snake-venom phosphodiesterase, however, the classification of Kunitz was found to be inadequate.
Briefly, extracted DNA was hydrolyzed with micrococcal nuclease
, spleen phosphodiesterase, and nuclease P1.
In the second stage this immune-selected chromatin bound to agarose is subjected to non-specific fragmentation either using sonication or mild micrococcal nuclease
In the current study, we set out to develop a set of synthetic cfDNA quality control materials (SCQCMs) that comprises spike-in cfDNA based on micrococcal nuclease
(MNase) digestion and matched genomic DNA.