The following strain specific positive controls, for the experiments without metabolic activation, were used to demonstrate the effectiveness of the test: 4-Nitro-1,2-phenylenediamine (NPD) (4 [micro]g/plate) was used for TA98, sodium azide (SAZ) (2 [micro]g/plate) for TA100 and TA1535,9-aminoacridine (9-AA) (50 [micro]g/plate) for TA1537, and methyl methanesulfonate
(MMS) (2 [micro]g/plate) for WP2.
Population pharmacokinetics of colistin methanesulfonate
and formed colistin in critically ill patients from a multicenter study provide dosing suggestions for various categories of patients.
Fish were euthanized using an overdose of tricane methanesulfonate
(MS-222) of a purity of at least 98% (1 g: 5,000 ml seawater).
Chemical, reagents and instruments Hydrogen peroxide (35%), titanium dioxide (p25 degussa), methyl methanesulfonate
(99%), Cyclophosphamide (CAS Number 6055-19-2), Triton X-100 (CAS Number 9002-93-1), acetone ([greater than or equal to]99.5%), ethanol ([greater than or equal to]99.8%), NaCl ([greater than or equal to]99.5%), KH2PO4 ([greater than or equal to]99%), Na2HPO4 ([greater than or equal to]99%), KCl ([greater than or equal to]99%), NaOH ([greater than or equal to]98%) and HCl (37%) were purchased from Sigma-Aldrich.
After 30 and 60 days, selected fish were anaesthetized with 100 mg/L MS-222 (C10H15NO5S; 3-aminobenzoic acid ethyl ester methanesulfonate
) (Feng et al., 2011).
At the end of the experiment, after fish were starved for 12 hours, randomized two fish per tank that were anesthetized with tricaine methanesulfonate
cordifolia stem's methanol extract against the mice erythrocytes using peripheral blood micronucleus assay and to assess the antigenotoxic and protective properties of the extract against methyl methanesulfonate
(MMS)-induced genotoxicity in mice utilizing the same assay.
To obtain a positive control, some glass slides were also exposed to ethyl methanesulfonate
(EMS) at a concentration of 5 mM for 24 hours.
(EMS) was used as positive control.
CP: 3 [micro]g/mL cyclophosphamide, MMS: 3 [micro]g/mL cyclophosphamide, and 10 [micro]g/mL methyl methanesulfonate
. n = 3.