We applied the following five cell counter- based formulas: F1, Shine and Lal Index: MCV x MCV x MCH/100 (values_-than 1530 in IDA); F2, Mentzler Index: MCV/RBC count; F3, Srivastava Index: MCH/RBC count_ (values than 13 Iron Deficiency anaemia; F4, England and Fraser Index: MCV- (5 x Hemoglobin) - RBC (Negative in thalassaemia trait and positive in IDA) ; F5, Ricerca Index: RDW/RBC count.