Additionally, these markers were tested for deviation from expected Mendelian ratios
by back-crossing F2 progeny to F1 parents and most segregated in a 1:1 ratio (Stratikopoulos et al.
In both RO and SR, the sex-ratios were close to the Mendelian ratio
(p>0.05), as also observed by Goes and Fransozo (2000) for the same specie, as well captured in Ubatuba.
The results suggest that the observed numbers are in line with the expected Mendelian ratio
. The degrees of freedom (df) are 3 and the probability P([X.sup.2] [greater than or equal to] 0.37) = 0.9463 on 3 degrees of freedom.
In all but 1 of 10 Fe families derived from bright-orange [F.sub.0] grandparents, the observed frequencies of orange progeny were consistent with Mendelian ratios
expected from a l-locus 2-allele system in which the brownish purple genotype was recessive.
In 17 broods, the mothers and spiderlings were all of the same genotype (AA), making it impossible to test for deviations from Mendelian ratios
among progeny genotypes.
Chi-square analysis indicated that 259 (91.8%) segregated according to the Mendelian ratio
(1:1), and 23 (8.24%; 17 in male and 6 in female) showed significant segregation distortion (P < 0.05).
All 9 volunteer plants from the part of Field 1 sown with glufosinate-tolerant variety gave high percentages of glyphosate tolerance offspring, but only 7 out of the 9 showed the expected 3:1 Mendelian ratio
, or more accurately, failed to deviate from it.
Chi-square analysis indicated that CN005, CN026, CN028, CN003, CN013, and CN067 were not in agreement with Mendelian ratios
(P < 0.05).
The expected ratio for the lack of transmittance via one of the gametes, would be 1:1 across the seed collected from the [R.sub.1] siblings, while a chimeric [R.sub.0] plant would result in [R.sub.2] individuals segregating in a Mendelian ratio
In crosses with at least two parents, a deviation from the expected Mendelian ratio
was observed, associated with deficiency of tolerant segregates.
The choice of approach has depended on the pattern of segregation of disease--whether consistent with Mendelian ratios
or polygenic complexity.
For each segregating marker, a chi-square analysis ([Alpha] = 0.01) was used to test for deviations from the expected Mendelian ratios
(1:1 in a the DH population, 1:2:1 or 3:1 in the [F.sub.2:3] population with codominant or dominant markers, respectively).