The mcrA gene fragments were amplified using the primer combinations MLf (5'-GGTGGTGTMGGATTC ACACARTA YGCWACAGC-3') and MLr (5'-TTCATTGCRTAGTTWGG RTAGTT-3'), yielding ~490-bp amplicons ; ME1 (5'-GCM ATGCARATHGGWATGTC-3') and ME2 (5'-TCATKGCRTA GTTDGGRTAGT-3'), yielding ~740-bp amplicons ; and MR1 (5'-GACCTCCACTWCGT VAACAACGC-3') and ME2, yielding amplicons of ~1,100 bp .
The mcrA gene, which is involved in methane production in methanogens, will be used in qPCR to examine the methanogen quantity present in vertical depth profile lake column samples taken from approximately the deepest point of each lake.
For example, methyl coenzyme M reductase A genes (mcrA) from Bathyarchaeota (formerly known as Miscellaneous Crenarchaeota Group) and from the new archaeal phylum Verstraetearchaeota were recently found, indicating their involvement in methane metabolism [37, 38].
Existing models such as FACET and MCRA (discussed in "Chemical Transfer to Food via Storage, Processing, and Packaging") could also be used to predict the transfer of chemicals to processed and packaged foods (Oldring et al.
A state inmate brought an action against employees of a former prison medical provider, alleging under [section] 1983 that the employees were deliberately indifferent to his serious medical needs in connection with his ongoing treatment for a variety of ailments, including severe back pain, migraine headaches, and hepatitis B and C, and that the employees violated the Massachusetts Civil Rights Act (MCRA).