Maxam-Gilbert sequencing

Maxam-Gilbert sequencing

A method for sequencing DNA which was described by Maxam and Gilbert.
References in periodicals archive ?
Sanger and Maxam-Gilbert sequencing technologies were the most common sequencing technologies used by biologists until the emergence of a new era of sequencing technologies opening new perspectives for genomes exploration and analysis.
Sanger and Maxam-Gilbert sequencing technologies were classified as the First Generation Sequencing Technology [10,16] who initiated the field of DNA sequencing with their publication in 1977.
Maxam-Gilbert sequencing reactions were carried out by the standard procedures [19].
The resulting copies were either cleaved into shorter segments (as in the case of Maxam-Gilbert sequencing) or prematurely terminated by reaction (as with Sanger sequencing) and the resulting collection of fragments were separated according to length using gel electrophoresis.
Maxam-Gilbert sequencing (40) was developed at Harvard in the United States of America, while the Sanger method (11) was developed at the MRC Laboratory for Molecular Biology at Cambridge in Britain.