Changes in signal molecules and maturation promoting factor
levels associate with spontaneous resumption of meiosis in rat oocytes.
In addition, some events during bovine oocyte maturation including mitogenesis and metabolism are regulated by cytosolic kinase activity such as MAPK also called "extracellular signal-regulated kinase" (ERK) and maturation promoting factor
However, it may be possible that GVBD is required for the full activation of MAPK: a positive feedback loop occurs between MAPK and maturation promoting factor
, (87,88) the activation of which is exactly analogous to the relation between MAPK and GVBD.
This study proves GFF role in activating Maturation Promoting Factor [MPF] through the Mitogen-Activated Protein Kinase [MAPK] so GFF has the opportunity for supplementation of oocyte maturation medium .
The process of meiotic maturation of oocytes involves activation of various signal transduction pathways to activate the Maturation Promoting Factor [MPF], which consists of the catalytic subunits, namely p 34cdc2 and regulatory subunits of cyclin-B.
Purified maturation promoting factor
contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2.
These treatments are commonly followed by the application of an inhibitor of protein phosphoiyiation (6-dimethylaminopurine; 6-DMAP) which prevents Maturation Promoting Factor
(MPF) activation or an inhibitor of protein synthesis (cycloheximide, CHX) that prevents cyclin synthesis (6-9).