Estao incluidos patogenos virais de significante importancia humana e veterinaria, tais como, Crimean-Congo hemorrhagic fever virus (CCHFV), Rift Valley fever virus (RVFV), Akabane virus (AKAV), Nairobi sheep disease virus (NSDV), Aino virus (AINV) e Peaton virus (PEAV) (4), assim como virus ja isolados na Amazonia Brasileira como: Guaroa virus (GROV), Maguari virus
(MAGV), Tacaiuma virus (TCMV), Guama virus (GMAV), Caraparu virus (CARV), Oropouche virus (OROV), Catu virus (CATUV), Icoaraci virus (ICOV) e Belem virus (BLMV), entre outros (2).
Maguari virus (MAGV) was first isolated from a mixed pool of mosquitoes collected in Utinga forest in Brazil in 1957 and has since been isolated from a variety of mosquito species (including Aedes spp., Anopheles spp., Culex spp., Wyeomyia spp., and Psorophora spp.) in Ecuador, Brazil, Trinidad and Tobago, Colombia, Argentina, and French Guiana (1).
CVV, Cache Valley virus; FSV, Fort Sherman virus; MAGV, Maguari virus; PLAV, Playas virus; TLAV, Tlacotalpan virus.
CVV, Cache Valley virus; CODV, Cordoba virus; MAGV, Maguari virus.
has been previously isolated from mosquitoes of the Aedes, Mansonia, and Psorophora spp.
A subset of serum samples with antibodies that neutralized CVV were titrated and further analyzed by PRNT by using CVV, CHLV (strain CHLV-Mex07), KRIV (strain KRIV-Mex07), SOURV (strain NJO-94f), Maguari virus (strain BeAr7272), and Wyeomyia virus (strain prototype).
Antibodies that neutralized Maguari virus or Tensaw virus were detected in 71/-300 humans in Florida in the 1980s (9); as observed in our study, the highest PRNT titers for most of the seropositive persons in that study did not exceed 40.
In addition, a 694-base fragment amplified from the RNA-M segment of WI-03BS7669, followed by nucleic acid sequence analysis and a BLAST search, showed 98% sequence identity with 6 CVV strains but only 77% identity with Maguari virus