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Estao incluidos patogenos virais de significante importancia humana e veterinaria, tais como, Crimean-Congo hemorrhagic fever virus (CCHFV), Rift Valley fever virus (RVFV), Akabane virus (AKAV), Nairobi sheep disease virus (NSDV), Aino virus (AINV) e Peaton virus (PEAV) (4), assim como virus ja isolados na Amazonia Brasileira como: Guaroa virus (GROV), Maguari virus (MAGV), Tacaiuma virus (TCMV), Guama virus (GMAV), Caraparu virus (CARV), Oropouche virus (OROV), Catu virus (CATUV), Icoaraci virus (ICOV) e Belem virus (BLMV), entre outros (2).
Maguari virus (MAGV) was first isolated from a mixed pool of mosquitoes collected in Utinga forest in Brazil in 1957 and has since been isolated from a variety of mosquito species (including Aedes spp.
CVV, Cache Valley virus; CODV, Cordoba virus; MAGV, Maguari virus.
Maguari virus has been previously isolated from mosquitoes of the Aedes, Mansonia, and Psorophora spp.
A subset of serum samples with antibodies that neutralized CVV were titrated and further analyzed by PRNT by using CVV, CHLV (strain CHLV-Mex07), KRIV (strain KRIV-Mex07), SOURV (strain NJO-94f), Maguari virus (strain BeAr7272), and Wyeomyia virus (strain prototype).
Antibodies that neutralized Maguari virus or Tensaw virus were detected in 71/-300 humans in Florida in the 1980s (9); as observed in our study, the highest PRNT titers for most of the seropositive persons in that study did not exceed 40.
In addition, a 694-base fragment amplified from the RNA-M segment of WI-03BS7669, followed by nucleic acid sequence analysis and a BLAST search, showed 98% sequence identity with 6 CVV strains but only 77% identity with Maguari virus (4).
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