gene mapping

(redirected from Macrorestriction)
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ge·net·ic map

an abstract representation of the ordered array of genetic loci such that the interval between entries has algebraic signs and magnitude proportional to the expected number of crossings over between them and distances are algebraically additive; for example, on a genetic map the combined distance between locus A and locus C is the algebraic sum of the two distances between loci A and B, and B and C.

gene mapping

Determining the location of hereditary information carried on chromosomes. In humans, this requires determining the base pairs (chemical code) of each of the estimated 20,000 to 25,000 genes. Once a gene is mapped, that information may be used to compare abnormal genes with normal ones. Molecular biological techniques may then be used to search for methods of treating and preventing conditions resulting from genetic abnormality.
Synonym: genome mapping; linkage map See: gene splicing
See also: mapping
References in periodicals archive ?
Similar to macrorestriction mapping, it provides lengths for long restriction fragments (13-15).
With regard to epidemiological findings, molecular typing using PFGE of macrorestriction fragments following digestion by Xba I endonuclease producing 25-35 fragments in the 10 -30 kb range has been used by Belgian and French workers to good effect.
Figure 3, panel A, shows the PFGE DNA macrorestriction band patterns of the serotype IV prototype isolates, designated 37, 38, and 39 (16), and profiles we classified as 39c and 39a from 2 study isolates expressing only C-a protein.
PulseNet standardized protocol for subtyping Listeria monocytogenes by macrorestriction and pulsed-field gel electrophoresis.
This clone was not typable by pulsed-field gel electrophoresis with SmaI macrorestriction digestion and belonged to multilocus sequence type (ST) 398 (1).
Macrorestriction with the XbaI enzyme discriminated the isolates into 23 distinct profiles (pulsotypes), designated I1-I23.
This network is based on characterization of whole bacterial genomes by using macrorestriction digestion patterns that are separated by pulsed-field gel electrophoresis (PFGE), a technique that has emerged as a common standard for subtyping EHEC O157 isolates (6).
Isolates were typed by using ApaI macrorestriction analysis and PFGE according to the manufacturer's recommendations (Bio-Rad, Marnes-la-Coquette, France).
Isolates were genotyped by pulsed-field gel electrophoresis after SmaI macrorestriction, spa sequence typing (http://spaserver.
Phenotypic tests and 16S rRNA gene sequencing showed that the penguin isolates were identical to each other, and macrorestriction profiling of pulsed-field gel electrophoresis fragments showed that they were very similar to fragments isolated from poultry in Washington in 1984 (1).
striatum isolates, we used 2 DNA fingerprinting techniques: automated ribotyping (RiboPrinter Microbial Characterization System; DuPont Qualicon, Wilmington, DE, USA) with EcoRI as restriction enzyme and pulsed-field gel electrophoresis (PFGE) macrorestriction analysis with 2 enzymes (XbaI and SwaI; New England Biolabs, Beverly, MA, USA).