Similar to macrorestriction
mapping, it provides lengths for long restriction fragments (13-15).
With regard to epidemiological findings, molecular typing using PFGE of macrorestriction
fragments following digestion by Xba I endonuclease producing 25-35 fragments in the 10 -30 kb range has been used by Belgian and French workers to good effect.
Figure 3, panel A, shows the PFGE DNA macrorestriction
band patterns of the serotype IV prototype isolates, designated 37, 38, and 39 (16), and profiles we classified as 39c and 39a from 2 study isolates expressing only C-a protein.
PulseNet standardized protocol for subtyping Listeria monocytogenes by macrorestriction
and pulsed-field gel electrophoresis.
This clone was not typable by pulsed-field gel electrophoresis with SmaI macrorestriction
digestion and belonged to multilocus sequence type (ST) 398 (1).
analysis of the 4 isolates showed that K.
with the XbaI enzyme discriminated the isolates into 23 distinct profiles (pulsotypes), designated I1-I23.
This network is based on characterization of whole bacterial genomes by using macrorestriction
digestion patterns that are separated by pulsed-field gel electrophoresis (PFGE), a technique that has emerged as a common standard for subtyping EHEC O157 isolates (6).
Isolates were typed by using ApaI macrorestriction
analysis and PFGE according to the manufacturer's recommendations (Bio-Rad, Marnes-la-Coquette, France).
Isolates were genotyped by pulsed-field gel electrophoresis after SmaI macrorestriction
, spa sequence typing (http://spaserver.
Phenotypic tests and 16S rRNA gene sequencing showed that the penguin isolates were identical to each other, and macrorestriction
profiling of pulsed-field gel electrophoresis fragments showed that they were very similar to fragments isolated from poultry in Washington in 1984 (1).
striatum isolates, we used 2 DNA fingerprinting techniques: automated ribotyping (RiboPrinter Microbial Characterization System; DuPont Qualicon, Wilmington, DE, USA) with EcoRI as restriction enzyme and pulsed-field gel electrophoresis (PFGE) macrorestriction
analysis with 2 enzymes (XbaI and SwaI; New England Biolabs, Beverly, MA, USA).