gene mapping

(redirected from Macrorestriction)
Also found in: Dictionary.

ge·net·ic map

an abstract representation of the ordered array of genetic loci such that the interval between entries has algebraic signs and magnitude proportional to the expected number of crossings over between them and distances are algebraically additive; for example, on a genetic map the combined distance between locus A and locus C is the algebraic sum of the two distances between loci A and B, and B and C.

gene mapping

Determining the location of hereditary information carried on chromosomes. In humans, this requires determining the base pairs (chemical code) of each of the estimated 20,000 to 25,000 genes. Once a gene is mapped, that information may be used to compare abnormal genes with normal ones. Molecular biological techniques may then be used to search for methods of treating and preventing conditions resulting from genetic abnormality.
Synonym: genome mapping; linkage map See: gene splicing
See also: mapping
References in periodicals archive ?
maculicola M2 macrorestriction pattern of DNA chromosome with PacI, PmeI and SwaI
Fiveteen different macrorestriction patterns could be differentiated by the macrorestriction analysis of the 27 S.
Similar to macrorestriction mapping, it provides lengths for long restriction fragments (13-15).
aeruginosa isolates by pulsed-field gel electrophoresis (PFGE) following chromosomal macrorestriction has been shown to be epidemiologically useful for strain differentiation, [11-13] and this technique was employed to investigate P.
Nosocomial colonization and infection with multiresistant Acinetobacter baumannii: outbreak delineation using DNA macrorestriction analysis and PCR-fingerprinting.
aureus strains by PCR for rRNa gene spacer size polymorphism and comparison to Smal macrorestriction patterns.
PulseNet standardized protocol for subtypingL/sfena monocytogenes by macrorestriction and pulsed-field gel electrophoresis.
b) Analysis of the macrorestriction profiles of chromosomal DNA after cleavage with the restriction enzyme SmaI and pulsed field electrophoresis.
The profile analysis by PFGE macrorestriction and the results obtained with the restriction enzyme ApaI (Figure 1) allowed the distribution of the seven strains in one cluster (>80% similarity), two pulsotypes (A and B) and two subtypes (A1 and B1).
Macrorestriction polymorphism of genomic DNA determined by PFGE revealed the presence of two pulsotypes among the nine study isolates.
We used convenience sampling to select 62 CPRKp isolates that were detected during 2014-2015 and used pulsed-field gel electrophoresis (PFGE) to evaluate their genomic DNA macrorestriction profiles after XbaI digestion.
The clonal relatedness was investigated by pulsed-field gel electrophoresis (PFGE) with SmaI macrorestriction according to standard procedures.