fco 500 g,mueller hinton agar 500g fco,1 microliter calibrated loop p / des urine culture.
The results showed that APAS achieved over 98% sensitivity in its detection of disease-causing bacteria on two of the most widely-used culture media -- Blood agar and MacConkey
agar -- in urine samples from 5,500 patients.
The isolate grew on blood, MacConkey
, and chocolate agar when subcultured and was oxidase positive.
For this study, a sample of each stored strain was checked for purity on MacConkey
agar and kept at -20[degrees]C in Tryptic Soy Broth (TSB, Difco Laboratories, USA) with 15% glycerol.
The culture media used included nutrient agar, MacConkey
agar and chocolate agar.
coli O157:H7 should be cultured using MacConkey
agar because this particular strain does not ferment sorbitol.
Viable counts of bacteria in the fecal samples were then determined by plating serial 10-fold dilutions (in 1% peptone solution) onto MacConkey
agar plates (Difco Laboratories, Detroit, MI, USA) and lactobacilli medium III agar plates (Medium 638, DSMZ, Braunschweig, Germany) to isolate Escherichia coli (E.
coli O157:H7 counts were determined for each manure types weekly by culturing on selective agar medium--Sorbitol MacConkey
agar (March and Ratnam, 1986) and then confirmed both biochemically and serologically as described by Cheesbrough (1991) and Nataro and Kaper (1998) respectively.
The yolk sac fluid was plated on selective MacConkey
Specimens were then identified using criteria of colony morphology of common enteric bacteria on differential and selective plating media; for example, MacConkey
agar with crystal violet, XLD agar, SS agar, DCA, and HEA.
CC] were added to macconkey
sorbitol agar and cultured as sameness.
Following the morphological studies of bacterial colonies and cells, the biochemical properties of unknown bacteria were explored through their growth and colony color on two selective and differential media, MacConkey
agar and mannitol salt agar, and the results of the oxidase test.