Briefly, samples were smeared on blood agar and MacConkey
agar plates and incubated at 37AdegC for 24 hours.
The authors stated that multiple agars such as MacConkey
and blood were used to inoculate bacteria, albeit, all samples were later incubated aerobically for 24 hours at37AdegC.1 However, this technique would havecompletely destroyed anaerobes, which can be the potential causative agents in this case.
Samples were processed and cultivated under aerobic and microaerobic conditions in 5% sheep blood agar and MacConkey
agar, and incubated for 72h at 37[degrees]C.
mirabilis, Whitby, et al., 1945; MacConkey
, et al., 1999; Sokokski, and Stapert, 1963 and Williams, 1973, have been used numerous media to prevent this swarming, among these are very dry plate, MacConkey
medium containing bile salt, ferrous ions and P-nitrophenyl glycerin.
Surface samples were collected from 3 points in hospital rooms (floor, bed footboard, and sink) as previously described (9), then grown in MacConkey
broth for 48 h at 37[degrees]C to amplify the Enterobacteriaceae population.
The other portion of the sample was inoculated on Nutrient, Blood and MacConkey
agar media and incubated aerobically at 37[degrees]C for 24 hours.
Then they were enumerated onto a culture medium of 1% L-Rhamnose MacConkey
The organisms were inoculated on MacConkey
agar and EMB agar and identified via biochemical tests.
Another study (3) with more 5,000 samples looked at the use of algorithms on blood and MacConkey
plates and showed agreement between the software and manual interpretation was 99.96 percent for positive, 92.6 percent for negative.
After approximately 24 hours of incubation, two colony morphologies were observed; one proved to be P aeruginosa, the other, appeared as a round, convex nonlactose fermenting organism, which grew well on 5% sheep blood, chocolate, and MacConkey
Two culture media were used in this experiment: blood agar (Oxoid Ltd., Basingstoke, and Hampshire, United Kingdom) and MacConkey