However, engineering chickens with an "always-on" Mx gene
might be detrimental, so the strategy would be to make expression of the gene contingent on influenza infection.
With recombinant DNA techniques, the scientists have transferred many copies of a normal Mx gene
into cells of susceptible animals.
Mx gene expression under different conditions in vivo and in vitro was also conducted.
The structure of the chicken Mx gene was deciphered by screening a chicken genomic bacterial artificial chromosome (BAC) library followed by sequencing of the specific insert.
Real-time PCR results of the expression level of Mx gene in different tissues was identical to semi-quantitative RT PCR (Figure 1B).
All polyI:C doses resulted in significantly increased Mx gene expression compared with the untreated control.
The frequency of the resistant allele was also found to be significantly different between native and highly selected commercial chicken lines, indicating the Mx gene could be positively selected in native chicken breeds due to its resistant activity (Li et al.
The genotypes of the chickens were screened using PCR single strand conformation polymorphism (PCRSSCP) for the S631N position of the Mx gene.
The standard deviations of antibody levels in all groups were high, which indicates that there could be many genes associated with antibody response to AIV besides the Mx gene.
We did not examine the disease resistant activities to AIV in the chickens because the chickens were randomly selected and also the anti-disease activities of the Mx gene had been only found in vitro (Ko et al.
However, until now no evidence of association between polymorphisms and economic traits in the porcine Mx gene
has been reported.
A number of IFN-stimulated genes (ISGs), including the MX genes
, are expressed in the uterus (Spencer et al.