Therefore, the castration performed in the present study and the resistance training was to relate the effects of the absence of testosterone and training in Wistar rats on the gene expression of MSTN
A, Protein content of ubiquitinated (UB) proteins, total LC3 and form II, and GABARAP from cardiomyocytes incubated with myostatin (MSTN
) (100 ng/mL) for 24 h with densitometry analysis of these proteins corrected to b-actin.
Therefore, in this study we used 17-week-old Sansui ducks to study the association of single nucleotide polymorphisms (SNPs) in MSTN
exon and slaughter traits.
The inhibitory effect of ActRIIB-ECD on MSTN
activity was shown in vitro in A204 cells, using purified, recombinant saActRIIB-l-ECD (sa, from Sparus aurata) produced in the yeast Pichia pastoris and the CAGA-luciferase reporter gene assay [which uses a plasmid consisting of 12 Smad3/Smad4 binding sequences (CAGA boxes) and the luciferase-coding sequence] (Funkenstein et al., 2012).
It should be mentioned that the Hardy-Weinberg equilibrium for MSTN
gene in this population was at odds with the results reported by other researchers .Farhadiyan et al 2011 in a study on Makui'ssheep in intron 1 were performed on 417 pieces deviation from Hardy Weinberg equilibrium in this population were reported .
ncbi.nlm.nih.gov), Gallus gallus MSTN
, NM_001001461 and UCSC Genomics Institute, chicken BLAT (http://genome.ucsc.edu)], the target guide RNA (gRNA) sites were designed following protospacer adjacent motif (PAM) sequences (Figure 1).
Above all, transcription of MSTN
, which was tightly related to muscle development, was upregulated in the muscle of crisp grass carp (Table 3).
(Zhang et al., 2014) successfully produced sheep fetal fibroblasts with a MSTN
disruption at the exon 1.
However, the lower prices for MSTN
and CSTN displays, still a large part of shipments from China, skewed revenue shares.
) is a transforming growth factor (TGF)-[beta] superfamily member that plays an important inhibitory role during muscle development (McPherron et al., 1997).
Previous genome-wide association studies (GWAS) have identified a handful of genetic markers in the myostatin (MSTN
) (Grobet et al., 2003) with racing performance (Binns et al., 2010; Hill et al., 2010; Tozaki et al., 2010).