(1), on the other hand, reported that neither MRP8 or MRP14 was detectable in resident macrophages in sections of healthy human tissue, but in acutely inflamed tissue, such as in gingivitis, psoriasis, neurodermatitis, and erythrodermia, MRP14 was present in macrophages of the perivascular infiltrate in 15 of 32 cases analyzed, whereas MRP8 was always absent.
In addition, MRP8 and MRP14 proteins were purified electrophoretically.
The MRP8, MRP14, and MRP8/14 proteins, which were electrophoretically purified from 15% gels by SDS-PAGE in the absence of 2-ME, were used (see Fig.
Two subunits of MRP8 and MRP14 in the polyacrylamide gel were transferred to a PVDF membrane (Sequi-Blot[TM]; Bio-Rad Laboratories) and used for amino acid sequence analysis.
SDS-PAGE of MRP8, -14, and -8/14 was carried out in the absence (Fig.
In addition, both of the antibodies reacted with MRP8/14 protein specifically but not at all with the individual subunits, MRP8 or MRP14 (Fig.
On the other hand, MRP8/14 complex is likely to be simultaneously produced in human granulocytes in the acute inflammatory phase because MRPs (MRP8 and MRP14), particularly MRP14, are expressed exclusively in human activated granulocytes, mainly neutrophils, in response to acute inflammatory reaction at the early stage (1,13).
Some roles of MRP8 and MRP14 in the cells have been reported (6,11,14, 26), whereas those of the MRP8/14 complex are poorly understood.
Calcium-induced noncovalently linked terramers of MRP8 and MRP14 detected by ultraviolet matrix-assisted laser desorption.
Park, "MRP8 promotes Th17 differentiation via upregulation of IL-6 production by fibroblast-like synoviocytes in rheumatoid arthritis," Experimental & Molecular Medicine, vol.
Hynes et al., "A Role for MRP8 in in stent restenosis in diabetes," Atherosclerosis, vol.