An additional protein in the ABC transporter family, MRP3
, upregulates in cholestasis, protecting the hepatocytes from toxin-related damage and death.
Conjugated BS need to be actively transported into cholangiocytes via apical sodium-dependent bile acid transporter (ASBT, gene SLC10A2) and are exported into the peribiliary capillary plexus via the heterodimeric transporter OST[alpha]-OST[beta] and via MRP3
The relatively high concentration of UGT, MRP2 and ABCG2 in the initial parts of the intestine, (duodenum and jejunum) might form a first line of defense against ingested xenobiotic compounds, while the presence of MRP3
in later portions of the intestine (ileum and colon) may ensure the absorption of specific nutrients and beneficial compounds.
Fifth factor which now have left by only public distribution system (MRP4) were added by another two compartments which had loading onto this factor as well; different pricing mechanism (MRP5) & controlled price at milling (MRP3
Quantitative RT-PCR analyses revealed that EPA and/or DHA significantly downregulate the expression of genes controlling BA synthesis (CYP7A1 and CYP27) and uptake (NTCP), while genes involved in efflux (such as MRP2, MRP3
, MRP4, and OST[beta]) and metabolism (SULT2A1) were upregulated (Figure 1(a)).
After incubation with MK 571, which inhibits MRP3
on the basolateral side, the AP to BL transport of rutin also decreased significantly (43.27 [+ or -] 2.18 versus 79.38 [+ or -] 2.79 nmol/L x mg x protein, P < 0.01, n = 7) and increased in the BL to AP direction (153.75 [+ or -] 5.66 versus 135.23 [+ or -] 5.65 nmol/L x mg x protein, P < 0.05, n = 7).
ABC transporter BBB BSCB Localization in (gene) brain capillary endothelium P-gp (ABCB1) Yes  Yes Luminal  MRP1 (ABCC1) Yes [24, 25, 40] Yes Luminal  Abluminal  MRP2 (ABCC2) Yes [24, 27] Yes Luminal  MRP3
(ABCC3) Yes  ?
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Expression of drug resistance proteins Pg[micro]MRP1, MRP3
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