and mrp2 (Mg mrp1
and Mg mrp2), genes involved in the detoxification of okadaic acid in M.
The work horses for detoxification are 1) MRP1
, the transmembrane transporter serving most cells in the body for exporting toxins to circulation, 2) OATP, the basolateral membrane transporter which moves toxin conjugates from the blood into the hepatocytes or renal tubule epithelial cells, and 3) MRP2, the apical transporter that moves toxin conjugates (and some bile salts) into the bile canaliculus or renal proximal tubule lumen.
Antibodies against multidrug-resistance-associated protein 1 (MRP1
) (anti-MDR1 [U1C2]) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Souza e Silva et al., "MRP1
expression in CTCs confers resistance to irinotecan-based chemotherapy in metastatic colorectal cancer," International Journal of Cancer, vol.
The [DELTA][DELTA]Ct method was used to calculate MDR-1, CRT, and MRP1
gene expression levels relative to control and the housekeeping gene [micro]-actin was used for normalization of MDR-1 expression.
Klaassen, "Organ distribution of multidrug resistance proteins 1, 2, and 3 (Mrp1
, 2, and 3) mRNA and hepatic induction of Mrp3 by constitutive androstane receptor activators in rats," The Journal of Pharmacology and Experimental Therapeutics, vol.
The three most well-studied transporters known for their efflux capabilities include P-glycoprotein (P-gp; ABCB1), multidrug resistance-associated protein 1 (MRP1
; ABCC1), and breast cancer resistant protein (BCRP; ABCG2) [11, 21-24].
Multidrug resistance associated protein 1 and 2 (MRP1
and2)###Wortelboer et al.
Multidrug resistance proteins: role of P-glycoprotein, MRP1
, MRP2, and BCRP (ABCG2) in tissue defense.
Among those, we selected, for the microarray and RNA-seq analyses, four PG transporters, ABCC1 (also known as MRP1
), ABCC9 (also known as sulfonylurea receptor 2 [SUR2]), SLCO4C1 (also known as OATP4C1), and SLCO5A1 (also known as OATP5A1), because these have been shown to be differentially expressed in the uterine endometrium during early pregnancy [10,11].
The multidrug resistance protein-1 (MRP1
) was suggested as a mediator of the effect of LTC4 on atherosclerosis.
[sup.99m]Tc-MIBI is a substrate for PGP (P-glycoprotein, gene symbol ABCB1), MRP1
(multidrug resistance protein 1 (gene symbol ABCC1)), and MRP2 (multidrug resistance protein 2 (gene symbol ABCC2)) and can be used to image their expression in vivo .