With the exception of human and chicken erythrocytes, both MSHA and MRHA strains agglutinated the erythrocytes of horse, rats, sheep, and rabbit with varying haemagglutination capacity (HT=6-36).
Prior to haemagglutination testing, the trypsinization of the erythrocytes tested revealed increases in haemagglutination titres and the number of MSHA and MRHA strains of S.
The data presented in Table 3 revealed non-haemagglutination inhibitory effects of N-acetylgalactosamine, N-acetylglucosamine, D-glucose, D-galactose, D-lactose, and D-rhamnose on the agglutinability of MSHA and MRHA strains to guinea pig erythrocyte.
Although there is no clarification on the fimbrial origin of these adhesions, the presence of MSHA and MRHA in our strains corroborates the findings of the study that revealed and characterized cell-associated haemagglutinin in S.
Nevertheless, the ability of our MSHA and MRHA strains to display agglutinability to erythrocytes of rabbit, rat, chicken, horse, and sheep indicated broad-spectrum haemagglutinating activity of Shigella haemagglutinins.
The present study has demonstrated the presence of MSHA and MRHA with broad-spectrum haemagglutination reaction in Shigella strains in Nigeria.