CD99, chromogranin A, CD45, inhibin, calretinin, CA-125, ER, PR, CK7, CK20, Moc31
, Tag72, myogenin, S100, and destine were negative.
The EpCAM-CAR construct sequentially contains a mouse anti-human EpCAM scFv fragment (MOC31
), CD8 hinge and transmembrane domains, and the intracellular signaling domains of 4-1BB and CD3[zeta].
Histologic specimens obtained at surgery were fixed in 10% formalin, routinely processed and embedded in paraffin All specimens were evaluated immunohistochemically with antibodies calretinin, vimentin, CK5/6, MOC31
, CD15 and CEA using staining procedures.
The diagnostic utility of MOC31
, BerEP4, RCC marker and CD10 in the classification of renal cell carcinoma and renal oncocytoma: An immunohistochemical analysis of 328 cases.
Desmoplastic small round cell tumor shows a complex immunophenotype with common coexpression of epithelial (cytokeratins, epithelial membrane antigen, MOC31
, Ber EP4), muscle (perinuclear "dot" desmin), and neural (neuron-specific enolase, CD 57) markers.
EMA (Figure 1, d [red, with cell membrane accentuation]) indicates a malignant condition, whereas BerEp4 (Figure 1, c and j [brown]), carcinoembryonic antigen (CEA; Figure 1, h), MOC31
, thyroid transcription factor 1 (TTF1; Figure 1, i), CD15, and sialyl-TN are usually negative in MM and positive in metastatic adenocarcinoma.
Utility of WT-1, p63, MOC31
, mesothelin, and cytokeratin (K903 and CK5/6) immunostains in differentiating adenocarcinoma, squamous cell carcinoma, and malignant mesothelioma in effusions.
(2) Based on sensitivity and specificity, calretinin (Figure 8, A and B), cytokeratin 5 or 5/6 (Figure 9, A and B), WT1 (Figure 10, A through C), and podoplanin (D2-40) (Figure 11, A and B) are the best positive mesothelioma markers, whereas claudin 4 (Figure 12, A and B), MOC31
(Figure 13, A through C), and BER-EP4 are the best overall carcinoma markers.
Hepatoid carcinomas may also express MOC31
and mCEA, which are infrequently expressed in HCCs.
(17) In contrast, CA9, (13,19) MOC31
, (11) c-kit, (19) VEGFR-2, (19) PDGFR-[alpha], (19) and TFE (18) were not detected.
The most useful general carcinoma markers are MOC31
, BG8, carcinoembryonic antigen, and BerEp4.
Lung Adenocarcinoma Versus Mesothelioma (Second IHC Panel) Marker Lung Adenocarcinoma Mesothelioma Napsin A + - MOC31
+ - CEA + - CK5/6 - + Thrombomodulin -/+ + HBME1 -/+ + Abbreviations: CEA, carcinoembryonic antigen; CK5/6, cytokeratin 5/ 6; HBME1, Hector Battifora mesothelial/1; IHC, immunohistochemical; +, greater than 75% of cases are positive; -, fewer than 5% of cases are positive; -/+, fewer than 50% of cases are positive.