and CAM5.2, epithelial membrane antigen (EMA), CD31, and CD34 were also negative, as was CAMTA1, a recently described antibody expressed in epithelioid hemangioendothelioma (Table 1 and Fig.
Thus, an IHC panel of lowmolecular weight and high-molecular weight cytokeratins (including, but not limited to, 5/6, 7, CAM 5.2, K903, AE1/ AE3, and MNF116
) and, if negative, a second tier of myoepithelial markers, will often show positivity with at least 1 marker (Figure 3, B).
No specific reaction was found with desmin, CD34, Pankeratin MNF116
, and EMA.
Immunohistochemistry presented positivity for glial fibrillary acidic protein (GFAP), smooth muscle actin, cytokeratin (AE1AE3 and MNF116
), calponin, P63, S100, and CD99.
IHC analysis with antibodies to cytokeratins (MNF116
) facilitates the detection of isolated tumour deposits.
 found that pankeratin (MNF116
) is the most sensitive marker (93%), followed by cytokeratins 14 (90%), for identifying MpBC.
Positive controls were used as monoclonal antibodies against cytokeratins (clone MNF116
, DakoCytomation, Denmark).
The tumour cells were negative for cluster of differentiation (CD) 117, CD34, CD99, CD68, S100, Activin receptor-like kinase-1, MNF116
, and Human Melanoma Black -45.
Immunohistochemistry was performed, and the tumor cells showed negative expression for MNF116
, L26, and CD45RO (UCHLI).
(b) Cocktail consists of cytokeratin AE1/3 (1:60), MNF116
(1:60), and Cam 5.2 (1:100).
(1-4) Several authors have established that immunostains for cytokeratins (CKs), such as CK MNF116
and CK 5/6, as well as the myoepithelial cell marker p63, are variably positive in the spindle cells of metaplastic carcinomas and are helpful in supporting the diagnosis.
Squamous cell carcinoma with single cell infiltration: a potential diagnostic pitfall and the utility of MNF116