(2009) Variants within the MMP3
gene are associated with Achilles tendinopathy: possible interaction with the COL5A1 gene.
We evaluated the levels of MMP1, MMP2, MMP3
, and MMP9 using Western blotting.
Moreover, the Generalized Multifactor Dimensionality Reduction (GMDR, v0.9; http://www.ssg.uab.edu/gmdr/) [16, 17] analysis was used to calculate the interaction among five studied polymorphisms (-1607 1G/2G MMP1 (rs1799750), -735 C/T MMP2 (rs2285053), -1306 C/T MMP2 (rs243865), -1171 5A/6A MMP3
(rs35068180), and -1562C/T MMP9 (rs3918242)) in predicting of the occurrence of preeclampsia.
The following sequences were used for quantitative PCR (forward/reverse): ATP5O: 5'-AACGACTCCTTGGGTATTGCTTAA-3'/5'ATTGAAGGTCGCTATGCCACAG-3', ADIPOQ: 5'-TTTGGAGTGTTGGTAGGTGTCTGT-3'/5'GGGACATAGGTAAGAGGAAGTAGAGT-3', LIPE: 5'-GAAGACTCTGCAGGGATCCAATA-3'/5'TTTGGATGTAAGGTGATTGCTGTGG-3', Wingless-type family member 5A (WNT5A): 5'-CCAGTTCAAGACCGTGCAGAC-3'/5' CACACAAACTGGTCCACGATCTC-3', Fibroblast growth factor 5 (FGF5): 5'-CTTGAACAGCTGGAGGAATACATTTTA3'/5'TGGGACTTGGCATGGATAACTGTT-3', MMP1:5' -ACATGCGCACAAATCCCTTCTAC3'/5'CTTGGGGTATCCGTGTAGCA-3', MMP2: 5'-GGGACAAGAACCAGATCACATACAG-3'/5'CGAGCAAAGGCATCATCCACT-3', MMP3
: 5'-CTGCTTTGTCCTTTGATGCTGTC3'/5' AAACGAGGTCCTTGCTAGTAACTTCA-3'.
As for biomarkers evaluating the risk for cardiovascular events in diabetic patients, MMP3
, osteopontin and N-terminal prohormone of B-type natriuretic peptide (NT-proBNP), and their combined value score are currently proposed to be introduced in evaluating diabetic patients, but first there is a need for more clear evidence that their use offers a more accurate risk stratification than the traditional risk factors .
These studies are perhaps verified by cell culture analyses that have shown upregulation of ADEs such as NEP, ECE, ACE, ECE-2, IDE, and MMP2, MMP3
, and MMP9 on exposure to A[beta] [42, 43, 45-47].
The expression of matrix metalloproteinase 2 (MMP2), MMP3
, MMP13, a disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS4), ADAMTS5, interleukin 6 (IL6), C-C motif ligand 2 (CCL2), CCL5, and C-X-C motif ligand 10 (CXCL10) in NP cells was analyzed to investigate the SASP of rat NP cells.
The most important genes clustering CTCs in the subgroups were cadherin 2 (CDH2), collagen type I alpha 2 chain (COL1A2), collagen type V alpha 2 chain (COL5A2), fibronectin 1 (FN1), microtubule associated protein 1B (MAP1B), matrix metallopeptidase 3 (MMP3
), SRY-box 10 (S0X10), secreted phosphoprotein 1 (SPP1), zinc finger E-box binding homeobox 1 (ZEB1), and zinc finger E-box binding homeobox 2 (ZEB2; Fig.
The authors reported that Men-SCs were able to produce matrix metalloproteinase-3 (MMP3
), MMP10, granulocyte macrophage colony-stimulating factor (GM-CSF), angiopoietin-2, and platelet-derived growth factor (PDGF)-BB in quantities 10 to 10,000 times higher than those in umbilical cord blood cells.
Due to structural and substrate specificity, MMPs are currently divided into seven classes: collagenases (MMP1, MMP8, MMP13, and MMP18), gelatinases (MMP2 and MMP9), stromelysins (MMP3
, MMP10), stromelysin like (MMP11 and MMP12), matrilysins (MMP7 and MMP26), membrane type (MMP14, MMP15, MMP16, MMP17, MMP24, and MMP25), and others (MMP19, MMP20, MMP21, MMP22, MMP23, MMP27, and MMP28) [18, 27, 28].
En relacion con el grupo de las gelatinasas (MMP-2 y MMP-9), colagenasas (MMP-13) y estromelinas (MMP3
), autores como Iamaroon y colaboradores (36), Morris y colaboradores (23,24), Mansell y colaboradores (25), Blavier y colaboradores (37), Brown y colaboradores (38), de Oliveira y colaboradores (39), Gkantidis y colaboradores (40) e Hirata y colaboradores (41) han demostrado, al usar diferentes tecnicas de laboratorio, su implicacion en la palatogenesis en el raton.