Of the other four MMPs expressed at moderate levels in hMSCs at day 0 (MMP14, MMP16, MMP19, and MMP24), only MMP14 can degrade collagen type I, FN, and LN [30-32].
MMP16 was upregulated in 3D/Col1 bioprinted prisms.
Yu et al., "miR-146b-5p inhibits glioma migration and invasion by targeting MMP16
," Cancer Letters, vol.
To date, there are 28 MMP family members described, including the collagenase subfamily (MMP-1, MMP-8, and MMP-13), the gelatinase subfamily (MMP-2 and MMP-9), the stromelysin subfamily (MMP-3, MMP-10, and MMP11), membrane-associated MMPs (MMP-14, MMP-15, MMP16
, MMP-17, MMP-23, MMP-24, and MMP-25), and other MMPs, all of which possess a Zn ion-dependent endopeptidase activity homologue domain [18-20].
MMP2 (collagenase IV) and MMP16
(activates MMP2) expression was significantly upregulated at all time points, suggesting a detrimental role in wound healing processes .
Due to structural and substrate specificity, MMPs are currently divided into seven classes: collagenases (MMP1, MMP8, MMP13, and MMP18), gelatinases (MMP2 and MMP9), stromelysins (MMP3, MMP10), stromelysin like (MMP11 and MMP12), matrilysins (MMP7 and MMP26), membrane type (MMP14, MMP15, MMP16
, MMP17, MMP24, and MMP25), and others (MMP19, MMP20, MMP21, MMP22, MMP23, MMP27, and MMP28) [18, 27, 28].
MMPs can be divided into 4 main subtypes: collagenases (MMP-1, MMP-8, and MMP13), stromelysins (MMP-3, MMP-10, and MMP-11), gelatinases (MMP-2 and MMP-9), and membrane-type matrix metalloproteinases (MT-MMP, MMP-14, MMP-15, MMP16
, MMP-17, MMP-24, and MMP-25) .
Primers and probes were designed and validated by Applied Biosystems for 18SRNA (4308329), MMP1 (Hs00899658_m1), MMP2 (Hs00234422_m1), MMP3 (Hs00968308_m1), MMP8 (Hs01029057_m1), MMP9 (Hs00234579_m1), MMP13 (Hs00233992_m1), MMP14 (Hs00237119_m1), MMP16 (Hs00254755_m1) MMP24 (Hs00198580_m1), MMP25 (Hs01554789_m1), TIMP1 (Hs99999139_m1), and TIMP3 (Hs00165949_m1).
For MMP16, expression was not detected in the majority of the SG samples (17/22), except in 5/22 samples which presented low levels of this transcript; in GC, this protease was expressed at low levels in 8/17 samples, while the other 9/17 samples presented no expression.
hasmiR-546b-5p had inhibitory effect on pancreatic cancer cell migration and invasion by targeting MMP16
Genes for which some or all of the 267 measurements were nondetectable due to values <10 molecules/[10.sup.6] ACTB molecules included MMP16
, TPBG, LGALS9, CDC20, RAG1, and IFNG.
Membrane-anchored MMPs include MT1-MMP (MMP14), MT2-MMP (MMP15), MT3-MMP (MMP16
), MT4-MMP (MMP17), MT5-MMP (MMP24), MT6-MMP (MMP25), and MMP23.