Based on this, our study aimed to analyze the potential interference of administration of TA and PRP in culture of healthy chondrocytes, extracted from healthy metacarpophalangeal joints of horses, in relation to the gene expression of MMP13
and MMP9, IL-6 and ACAN.
Circular RNA related to the chondrocyte ECM regulates MMP13
expression by functioning as a MiR-136 'sponge' in human cartilage degradation.
MMP2 (rs243865, rs2285053, rs243847, (2012) (20) rs2287074, rs9923304, rs11639960) MMP3 (rs639752, rs650108, rs679620, rs522616) MMP9 (rs3918253, rs17576, rs17577) MMP13
(rs2252070) MMP14 (rs1042704) TIMP2 (rs9894526) Salazar-Pelaez et al.
A family of zinc-dependent endopeptidases known as the matrix metalloproteinases (MMPs), especially active MMP13
, then triggers the collagenase activity.
Metalloproteinases belong to a large family of zinc-dependent endopeptidases that include collagenases (e.g., MMP1, MMP8, and MMP13
), gelatinases (MMP2, MMP9), stromelysins (MMP3, MMP10), matrilysins (MMP7, MMP26), and transmembrane metalloproteinases types I and II.
It has been reported that upregulation of MMP-3 and MMP13
by IL-1 activates MAPK pathways as well as NF-[kappa]B transcription factors in articular chondrocytes .
Meanwhile, the mRNA expression levels of Acta2, MMP13
, Coll1a1, and TIMP1 were examined, which are all classical profibrogenic markers during liver fibrosis process [16, 17].
(9) Bonaldi and Morgia et al have also cited increased serum level of MMP13
in prostatic carcinoma patients in different studies.
Lopez et al., "Collagenase-3 (MMP13
) expression in chondrosarcoma cells and its regulation by basic fibroblast growth factor," The American Journal of Pathology, vol.
Hu et al., "Circular RNA related to the chondrocyte ECM regulates MMP13
expression by functioning as a miR-136 'sponge' in human cartilage degradation," Scientific Reports, vol.
miR-140 is also able to target ADAMTS5  and MMP13
 fundamental matrix-degrading enzymes in OA development.
Studies show that morin inhibits IL-1[beta]-induced phosphorylation of p38 and ERK1/2, decreases the expression of MMP-3 and MMP13
, and increases TIMP-1 expression .