Matrix metalloproteinases (MMPs), such as MMP1
and MMP2, are extracellular matrix (ECM)-degrading enzymes that function in the extracellular environment of cells to degrade both matrix and non-matrix proteins, [sup], and their activities are regulated by tissue inhibitor of metalloproteinases (TIMPs).
The MMP1-1607 (1G>2G) polymorphism has been associated with increased transcription of MMP1
due to an insert of a guanine base that creates a core-binding site for the EST family of transcription factors, which leads to increased susceptibility for tumor occurrence and progress.
Guo, "MiR-361-5p inhibits glycolytic metabolism, proliferation and invasion of breast cancer by targeting FGFR1 and MMP1
," Journal of Experimental & Clinical Cancer Research, vol.
Gene specific human Taqman[R] primers MMP1
(Hs00899659_m1), MMP13 (Hs00233992_ml), CCL2 (Hs00234140_m1) CCL8 (Hs04187715_m1), CXCL6 (Hs00605742_m1), C3 (Hs00163811_ml), CH25H (Hs02379634_s1), and LBP (Hs01084621_ml) (Applied Biosystems) were used for gene expression analysis.
Kimmel et al., "Agonist-like SERM effects on ERa-mediated repression of MMP1
promoter activity predict in vivo effects on bone and uterus," The Journal of Steroid Biochemistry and Molecular Biology, vol.
Likewise, IL-10 also appears to have an important role in modulating the healing process, since in vitro studies have demonstrated its role in the induction of collagen synthesis and action of enzymes that degrade collagen, such as MMP1
and MMP8 .
Torres et al., "Association between MMP1
and MMP9 activities and ICAM1 cleavage induced by tumor necrosis factor in stromal cell cultures from eutopic endometria of women with endometriosis," Reproduction, vol.
SCNN1A, ACCN5, EDNRA, NR3C2, NPPA, DSP, ADRA1B, ACE Cardiac stenosis EtOH-Fr SREBF1, PDE5A, MMP1
(includes EG:4312) Liver proliferation Imipramine CXCR4.CXCL12, C5 Tachycardia Imipramine GRIN2B, ADORA3, KCNH7, CHRNA7, KCNG2, KCNQ1, CHRNA5, ADORA1, CASQ2, ADRA1B, ADRB3, ADRA1D Kidney failure Imipramine EPO, ALB, EDNRA, FKBP1A, NR3C2, VDR, PPP3CA Glomerular injury EtOH-Fr SREBF1 Cardiac congestive Cardiac failure Imipramine EPO, NOS1.
For genotyping, we used previously published primer sets and PCR conditions for the matrix metalloproteinase-1 (MMP1
) (2), matrix metalloproteinase-3 (MMP3) (3), and plasminogen activator inhibitor-1 (PAI-1) (3) genes.
Circulating plasma levels of GDF15, matrix metalloproteinase 1 (MMP1
), soluble P-selectin (sP-selectin), and soluble tumor necrosis factor receptor II (sTNFRII) and serum levels of lipocalin 2 (LCN2), matrix metalloproteinase 2 (MMP2), and resistin were measured using commercially available ELISA kits (R&D, Minneapolis, MN, USA).
mRNA expression in BT-483 and MDA-MB-231 had significantly decreased in curcumin treatment group compared with control group.
We observed a twofold increase in TIMP1 and a threefold downregulation in MMP1
in ischemic WJ-MSCs, although there was an upregulation of MMP2 in the ischemic WJ-MSCs.