However, the median serum level of MMP-2
was statistically higher in controls as compared to OSCC patients thereby giving idea about some feedback regulation.
GPNMB regulated the expression and activity of MMP-2
and MMP-9 in cervical cancer cells
In male reproductive system, MMP-2
and MMP-9 are detected in seminal plasma.
The matrix metalloproteinases (MMPs) play an important role in the process of cardiac remodeling by regulating structural integrity of the extracellular matrix. MMP-2
is one of the predominant MMPs expressed in the cardiac ventricles.
There is a higher prevalence of studies on MMP-2
activation: to obtain active MMP-2
, 80 [micro]l of 0.7 mg/ml mouse MMP2/Tris-HCl solution was mixed with 0.1 ml of 2.5 mmol/l4-aminophenylmercuric acetate (APMA) and incubated for 2 h in a 37[degrees]C water bath.
Grzela, "Modulation of matrix metalloproteinases MMP-2
and MMP-9 activity by hydrofiber-foam hybrid dressing--Relevant support in the treatment of chronic wounds," Central European Journal of Immunology, vol.
, MT1-MMP, and TIMP-2 are essential for the invasive capacity of human mesenchymal stem cells: differential regulation by inflammatory cytokines," Blood, vol.
The pro-domains structure of MMP-1, MMP-2
, MMP-3 and MMP-9 has been well determined.
They are a family of zinc-containing enzymes capable of degrading components of ECM and connective tissues.[sup], MMP-2
is the most commonly expressed enzyme to degrade collagen, elastin, and fibronectin.[sup] Clinical studies revealed that the attenuation of MMP-2
expression was observed in plasma from patients with hypertension.[sup] Animal investigations from adult spontaneously hypertensive rats (SHRs) also indicated that pro-MMP2 and activated MMP-2
activities were diminished in mesenteric arteries, contributing to accumulation of collagen and fibronectin.[sup] The remodeling of large and small arteries may be the major causes of the development of ECM accumulation and hypertension.[sup]
The intensity of positive staining for collagen type I, MMP-2
, and MMP-9 was measured using Image-Proplus 6.0 software (Media Cybernetics, United States).
In the present study, we determined whether PNU-282987, a selective [alpha]7-nAChR agonist, affected activities of MMP-2
and MMP-9 and expressions of inflammatory cytokines MCP-1 and RANTES in nicotine treatment RAW264.7 and MOVAS cells.