Matrix metalloproteinases (MMPs) are special class of proteases that are categorized in five groups as per their substrate specificity: collagenases (MMP-1, -8 and -13), gelatinases (MMP-2 and -9), stromelysins (MMP-3, -10, and -11), matrilysins (MMP-7) and membrane-type (MMP-14
to -17, -24 and -25).
Twenty-four MMPs have been identified, including collagenases (MMP-1, 8, 13, and 18), gelatinases (MMP-2 and 9), stromelysins (MMP-3 and 10), matrilisins (MMP-7 and 26), and membrane-type MMPs (MMP-14
, 15, 16, 17, 24, and 25), among other types.
TIMP-3 expression was the highest in stable carotid plaques.[sup] Vulnerable plaque formation risk was significantly decreased in carotid plaque with MMP-14
position + 7096 TC + CC genotype compared to that in carotid plaque with MMP-14
Four of them are classified as type I membrane proteins, MMP-14
, MMP-15, MMP-16 and MMP-24, while remaining two (MMP-17 and MMP-25) are attached to the membrane by glycosylphosphatidylinositol molecule.
Based on the differences in their structure organization and substrate specificity, MMPs can been divided into 6 groups (2): 1) collagenases (MMP-1, 8, 13, and 18), of which the main function is degrading collagen types I, II and III; 2) gelatinases (MMP-2 and MMP-9), mainly acting on denaturing and cleaving type IV collagen and gelatin; 3) stromelysins (MMP-3, 7,10,11, 26 and 27), displaying hydrolysis ability of a broad range of ECM proteins, such as collagen types III, IV, V, elastin, proteoglycans and glycoprotein; 4) elastases (MMP-12); 5) membrane-type specific MMPs (MMP-14
, 15, 16, 17, 24, 25) with a furin cleavage site in the pro-peptide region that are mainly related to the activation of MMP-2; and 6) other MMPs (MMP-19, 20, 21, 22, 23, and 28).
These collagenases include MMP-1, MMP-8, MMP-13, and MMP-14
(MMP14 is a membrane-bound MMP) .
Tissue sections (7 pm) from middermal elastolysis or healthy control skins were used for immunohistochemical analysis of MMP-3, MMP9, MMP-2, MMP-1, TIMP-2, and MT1-MMP (MMP-14
MMP family members include collagenases (MMP-1, MMP-8, MMP-13, and MMP-18), gelatinases (MMP-2 and MMP-9), stromelysins (MMP-3, MMP-10, and MMP-11), matrilysins (MMP-7 and MMP-26), and membrane-type MMPs (MMP-14
Metalloproteinases include cell membrane metalloproteinases which are related to cell membrane (MMP-14
, MMP-15, MMP-16, and MMP-17), gelatinases (MMP-2 and MMP-9), stromelysins (MMP-3, MMP-7, MMP-10, MMP-11, and MMP-12), and collagenases (MMP-1, MMP-8, and MMP-13).
and TIMP-2 overexpression protects against hydroquinone-induced oxidant injury in RPE: implications for extracellular matrix turnover," Investigative Ophthalmology and Visual Science, vol.
The kit detects total activity of MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-12, MMP-13, and MMP-14
based on a 5-FAM/ QXL520 fluorescence resonance energy transfer (FRET) peptide, which is used as an MMP substrate.
PI3K inhibitor LY294002 (number 9901S), primary antibodies against Cox-2, PI3K, P-AKT, AKT, P-GSK3[beta], GSK3[beta], [beta]-catenin, MMP-2, MMP-7, and MMP-14
, and secondary antibody were all purchased from Cell Signaling Technology (Beverly, MA, USA).