meningitidis serogroup B stratified by sero-subtype were randomly selected and analysed by MLEE. In the collection of serogroup B isolates, 4 of the 13 enzyme loci were monomorphic and 9 were polymorphic for two to four alleles.
In both epidemics periods a clonal structure could be defined by means of MLEE, a characteristic of the population biology of this bacterium during epidemic waves of disease.
The MLEE results indicated that plants fell into two distinct lineages (I and II) separated by a genetic distance of 0.91 (Fig.
Interestingly, the MLEE data showed that this isolate (fourth from the top of Fig.
method used was as previously described (8-10); the electrophoretic mobility of 15 constitutive enzymes was analyzed.
Both serogroup A isolates from the 2000 outbreak were confirmed as subgroup III (ET-734, ST-5 with PFGE patterns characteristic for the "first pandemic wave" (14); serogroup W-135 isolates from the 2000 outbreak belonged to the ET-37 complex, as defined by MLEE
, and were designated as the (W)ET-37 clone (29).
There was a tremendous mlee
in the Plymouth area on 51 min-utes, when first Sugden and then Twiss had shots blocked, before Adams dispatched the ball to safety.
Multilocus enzyme electrophoresis (MLEE
) has been the reference method for global epidemiology of Neisseria meningitidis.
meningitidis have been assigned to a limited number of clonal groupings, historically by multilocus enzyme electrophoresis (MLEE
) (4) and currently by multilocus sequence typing (MLST) (6).
aureus, based on the classic multilocus enzyme electrophoresis (MLEE
) method used to study the genetic variability of a species.