The stimulation index is one of the formulas of the MLC test, and it is used for evaluation of matching between donor and recipient.
Stimulation index values in mMLC test were compared with stimula-tion index values in MLC test.
However, the MLC test fails to detect the minor histocompatibility antigens contributing to GVHD and tissue rejection and also the response stimulated by HLA-DP On the other hand, it is reported that GVHD and tissue rejection could be predicted by cytokine-modified mixed lymphocyte culture (mMLC) test (3-6).
In this study, cytokine-spiked mMLC test in addition to the MLC test were administered to patientdonor pairs.
The MLC test was administered in one-way and two-stage manner (recipient and donor) in the culture laboratory of the department.
On the MLC test, a SI value [less than or equal to] 1 indicates that the donor is not reactive to the recipient.
The mean age of the 59 patients who underwent MLC test and HSCT was 19.
The evaluation of the MLC test for SI in 59 patients revealed SI values [less than or equal to] 1 in 50 patients, while 9 patients had SI values > 1.
When the mean SI values obtained on the MLC and mMLC tests were evaluated with respect to GVHD development, the mean SI value of the 36 of the 59 patients undergoing MLC test who did not develop GVHD was 0.
The third modification was performed by re-addition of cytokines to the MLC test where stimulator cells were pre-treated with added cytokines.
15), exogenous cytokines were added to the one-way MLC test.
They found in that study that the MLC test was not adequate for predetermining acute GVHD; however, it could be the predictor of chronic GVHD when a result above Relative Response Index (RRI) = 4.