While global ABK inhibition in Drosophila S2 cells results in macro- and multi-nucleated large cells, mislocalization of midzone ABK (mABK) by depletion of Subito (Drosophila MKLP2) does not cause notable cytokinesis defects.
To better isolate the contribution of midzone ABK (mABK), the Drosophila mitotic kinesin-like protein 2 (MKLP2) homologue, Subito, which localizes chromosomal passenger complex (CPC) to the midzone in numerous model systems, including Drosophila (Gruneberg et al., 2004; Cesario et al., 2006; Nguyen et al., 2014), was depleted by RNAi.
While depletion of MKLP2 and Subito leads to loss of mABK in human and Drosophila cells, respectively, their depletion leads to a significantly higher incidence of cytokinesis failure in human cells (Zhu et al., 2005; Kitagawa et al., 2013, 2014).
Relocation of Aurora B from centromeres to the central spindle at the metaphase to anaphase transition requires MKlp2. J.
Targeting Aurora B to the equatorial cortex by MKlp2 is required for cytokinesis.