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(B) Proliferative marker MKi67 (red) by immunofluorescence in cross-sections from the control and genistein groups following 24-h treatment with vehicle, [E.sub.2], or [E.sub.2] and Dex.
(a) Correlation between CXCR4, CXCL12, VIM, CD163, and MKI67 in 315 luminal B (HER2-negative) primary BC.
Eight genes (EGER, MET, FLT1, PGR, PLAU, KIT, MKI67, and TWIST1) were significantly differentially expressed between those 2 groups (see Table 12 in the online Data Supplement).
In total, 14 genes including KRT19, FLT1, EGFR, EPCAM, MET, PGR, CD24, KIT, PLAU, ALDH1A1, CTSD, MKI67, TWIST1, and ERBB2 were evaluated as significantly differentially expressed in the tested cohorts between CTC+ and CTC- samples.
coli) MYC 2.04 V- 0.1 mycmyelocytomatosis viral oncogene homolog (avian) NOTCH 1 2.85 Notch 1 0.4 PRDM2 2.10 PR domain containing 1.3 2, with ZNF domain TUBS 1 1.58 Thrombospondin 1 4.4 TP53 2.05 Tumor protein p53 1.6 VEGFA 2.82 Vascular endothelial 0.07 growth factor A APC -3.79 Adenomatous 4.2 polyposis coli CCNDI -1.90 Cyclin D1 1.8 CCNEI -2.38 Cyclin E1 0.04 ESR1 -2.10 Estrogen receptor 1 1.9 IGFBP3 -2.58 Insulin-like growth 0.9 factor binding protein 3 KRTI9 -4.09 Keratin 19 0.06 MKI67 -1.51 Antigen identified 2.5 by monoclonal antibody Ki-67 MUC1 -1.59 Mucin 1, cell 2.2 surface associated PGR -3.09 Progesterone 3.0 receptor
A lower but not significant MKI67 protein expression was detected in western blot in DOCE+SIE group.
To investigate the effects on gene expression of 8-PN alone and in co-treatment with 3-MC, we chose genes that were identified in earlier experiments as being highly regulated by E2 (Areg, Csn2, Pgr) as well as genes associated with proliferation (Kif, Mki67; Figure 6C).
Genes associated with cell proliferation, such as marker of proliferation Ki-67 (MKI67) (also known as Ki67) and topoisomerase (DNA) II alpha 170kDa (TOP2A), are also highly expressed in basallike tumors.
Real-time PCR was performed to assess relative transcript levels of Cyp1a1, Cyp1a2, Cyp 1b1, Areg (amphiregulin), Ereg (epiregulin), Egf (epidermal growth factor), RacI (Ras related C3 botulinum substrate l),Tgfb (transforming growth factor beta), Bax (BCL-2 associate X protein), Ccndl (cyclin dl), Mki67 (antigen identified by monoclonal antibody Ki 67), Igft (insulin-like growth factor 1), Fgf2 (fibroblast growth factor 2), Ahr, Egfr (epidermal growth factor receptor), and Esrl (estrogen receptor, alpha) using Assays-on-Demand (Applied Biosystems), with Gusb (glucuronidase, beta) and Actb (actin, beta) as endogenous controls in hepatic and mammary tissues, respectively.
Survivin and MKI67 mRNAexpression in bladderwashings of patients with superficial urothelial cell carcinoma correlate with tumor stage and grade but do not predict tumor recurrence.
(a) Common Sequence ID Official gene gene name symbol BAG1 NM_004323 BAG1 Bcl2 NM_000633 BCL2 CCNB1 NM_031966 CCNB1 CD68 NM_001251 CD68 CEGP1 NM_020974 SCUBE2 CTSL2 NM_001333 CTSL2 EstR1 NM_000125 ESR1 GRB7 NM_005310 GRB7 GSTM1 NM_000561 GSTM1 HER2 NM_004448 ERBB2 Ki-67 NM_002417 MKI67 MYBL2 NM_002466 MYBL2 PR NM_000926 PGR STK15 NM_003600 AURKA STMY3 NM_005940 MMP11 SURV NM_001168 BIRC5 -Actin NM_001101 ACTB GAPDH NM_002046 GAPDH GUS NM_000181 GUSB RPLPO NM_001002 RPLP0 TFRC NM_003234 TFRC (a) Genes annotated with NCBI LocusLink sequence identification numbers and official gene symbols of the HUGO Gene Nomenclature Committee (http://www.gene.ucl.ac.uk/cgi-bin/nomenclature/searchgenes.pl).
The gene MK167 (proliferation marker antigen identified by the monoclonal antibody Ki-67) and the antiapoptosis gene survivin are promising prognostic markers, because increased MKI67 and survivin mRNA concentrations indicate short recurrence-free periods (1-4).
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