MIB1

MIB1

A monoclonal antibody which recognises a formalin-fixation-resistant epitope on the cell proliferation-associated Ki-67 antigen. MIB1 is a reliable marker of cell proliferation, and can be used for image analysis of paraffin-embedded tissues; Ki-67 is a short-lived nonhistone protein expressed during the G1, S and G2/M phases of the cell cycle, but not in the G0 (resting period) phase.

Image analysis of MIB1 may be an alternative method for objectively, reproducibly and reliably classifying dysplastic changes of the cervical epithelium. Its presence is regarded as an independent prognostic indicator in breast cancer—the presence of MIB-1 is associated with a slight increased tumour aggressiveness, but is a poor indicator of prognosis.
References in periodicals archive ?
The use of MIB1 to assess proliferative activity can occasionally be useful, particularly in spitzoid and other thick lesions, and we will invariably combine this with a Melan-A stain to help ensure that we are only assessing proliferative activity in melanocytes.
Aggressiveness and quantification of epithelial proliferation of middle ear cholesteatoma by MIB1. Laryngoscope 2003; 113: 328-31.
Uterine leiomyosarcomas are characterized by high p16, p53 and MIB1 expression in comparison with usual leiomyomas, leiomyoma variants and smooth muscle tumours of uncertain malignant potential.
Interplay among BRAF, p16, p53, and MIB1 in pediatric low-grade gliomas.
An immunohistochemical comparison between low-grade and high-grade ovarian serous carcinomas: significantly higher expression of p53, MIB1, BCL2, HER-2/ neu, and C-KIT in high-grade neoplasms.
In paraffin-embedded tissue, cells in active cell cycle phases can be visualized with the antibody MIB1 through a positive nuclear stain [6].
Hahka-Kemppinen et al., "MIB1 immunoreactivity correlates with blood vessel density and survival in disseminated malignant melanoma," Oncology, vol.
The CDKL5 substrates that have, so far, been identified are MeCP2 [11, 12], DNA methyltransferase 1 (DNMT1) [13], Rac1 [14], amphiphysin 1 [15], PSD-95 [16], NGL-1 [17], Mind Bomb 1 (Mib1) [18], Shootin1 [19], HDAC4 [20], and IQGAP1 [21].
The patient underwent complete resection of the mass at an outside institution and histologic examination of the lesion confirmed the presence of a WHO grade 1 meningioma with bland spindle cells with minimal mitosis and a MIB1 proliferative index of <3%.
Hall, "Detection of the Ki-67 antigen in fixed and wax-embedded sections with the monoclonal antibody MIB1," Histopathology, vol.
MIB1 proliferation index of [greater than or equal to]30% favors malignant potential [10].
Louis, USA), cytokeratin CAM5.2 (clone DC, 1:10, heat, Nichirei Bioscience, Tokyo, Japan), EMA(cloneE29,1:200, Agilent, SantaClara, CA, USA), PAX8 (polyclonal, 1:100, heat, Proteintech, Rosemont, IL, USA), S100 (polyclonal, Nichirei), HMB45 (clone HMB45, 1:50, heat, Leica), c-kit (polyclonal, heat, Agilent), CD10 (clone 56C6, 1:100, heat, Leica), MUC-1 (polyclonal, 1:200, heat, Leica), vimentin (clone SRL33, 1:200, heat, Leica), WT-1 (clone 6F-H2, 1:100, heat, Agilent), SMA (polyclonal, 1:300, Agilent), caldesmon (clone h-CD, 1:200, heat, Agilent), CD34 (clone NU-4A1,1:2, Nichirei), MIB-1 (clone MIB1,1:100, heat, Agilent), renin(cloneEPR20693,1:200, heat, Abcam, Cambridge, UK), and STAT6 (clone YE361,1:100, heat, Abcam).