Protein milk fat globule-EGF factor 8 (MFG-E8) was shown to recognize phosphatidylserine (PS) in the apoptotic neurons, thus enabling microglial phagocytosis .
M1 Polarized N9 Microglia Show Reduced Migration Ability Towards Chemoattractants but Display Increased Phagocytosis and MFG-E8 Expression.
In addition, we were able to observe, for the first time in this model, that 24 h incubation with LPS induces the expression and release of MFG-E8 from N9 cells (~1.5- and ~2-fold, respectively, p < 0.01), as indicated by Western blot analysis of total extracts and extracellular media (resp., Figures 4(c) and 4(d)).
More interestingly, we provide new evidence indicating decreased migration ability but increased phagocytosis with the involvement of MFG-E8 production and release.
A new finding was the increased expression of the phagocytosis-associated protein MFG-E8, together with its release, when the N9 cells were treated with LPS.
New findings also include the LPS-induced increase in the expression of MFG-E8, miR-155, and miR-146a, together with raised secretion of HMGB1 and MMP-9, as schematically represented in Figure 8.
Milk fat globule epidermal growth factor 8 (MFG-E8), a protein expressed mainly by activated macrophages and immature dendritic cells, is required for the phagocytosis of apoptotic cells as it links externalized phosphatidylserine present on the surface of apoptotic cells with integrins expressed on phagocytes (3).
The aims of this study were to investigate the levels of plasma MFG-E8 in patients with COPD and to analyze the potential association between MFG-E8, disease severity, and smoking history.
Plasma levels of MFG-E8, IL-1[beta] and TGF-[beta] were measured with commercial ELISA kits (MFG-E8, Cusabio, China, Cat.
Multiple linear regression was used to evaluate the relationship between the levels of plasma MFG-E8, different pulmonary function indicators, and confounding factors such as age, body mass index (BMI), and smoking history.
Plasma levels of MFG-E8 in COPD patients vs healthy controls
In the May 9 Nature, Shigekazu Nagata of Osaka University Medical School in Japan and his colleagues reported that lab-grown mouse macrophages secrete a sugar-laden protein called milk fat globule-EGF-factor 8 (MFG-E8
) that binds to cells that are killing themselves.