Rosengart, a team of researchers showed that administration of a cocktail made of transcription factors Gata4, Mef2c
and Tbx5 (GMT) resulted in less scar tissue, or fibrosis, and up to a 50 percent increase in cardiac function in small animal models of the disease.
To investigate the expression profiles of miR-127-3p during C2C12 cell proliferation, we determined expression of miR-127-3p and myogenic marker genes (MyoD, MyoG, Myf5, Mef2c, and Myosin) in C2C12 myoblasts in the proliferation medium for 0, 1, 3, 5, and 7 days by real-time RT-PCR.
Interestingly, we found that there were similar expression patterns between miR-127-3p and three myogenic marker genes including MyoD, MyoG, and Mef2c.
Then MEK5 and BMK1 are activated sequentially and BMK1 acts on its downstream targets including Mef2C
, c-Myc, and possibly Nrf2 (Figure 3).
Primers for the genes tested [Ahr, Cx40 (connexin 40), Cyplal (cytochrome P450 1A1), Ece1 (endothelin converting enzyme 1), Gata4 (GATA-binding protein 4), Gata6 (GATA-binding protein 6), Hcn4 (hyperpolarization-activated, cyclic nucleotidegated K+ 4), Kdr (kinase insert domain protein receptor), Mef2c (myocyte enhancer factor 2C), Mlc2v (myosin, light polypeptide 2, regulatory, cardiac, slow), Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha), Myh7 (myosin, heavy polypeptide 7, cardiac muscle, beta), Nanog (Nanog homeobox), Nkx2-5 (NK2 homeobox 5), Nppa (natriuretic peptide type A; Anf), Oct4 (POU domain, class 5, transcription factor 1; Pou5f1), Pgp9.
In mice, cooperative control of SMAD and WNT signaling pathways activates multiple transcription factors including Gata4, Nkx2-5, and Mef2c, which control cardiac differentiation (Pal and Khanna 2006).
and colleagues from UT Southwestern showed that four gene-regulatory proteins--GATA4, HAND2, MEF2C
, and TBX5 (GHMT)--can convert cardiac fibroblasts into beating cardiac-like muscle cells.
Utilizando un protocolo analogo al de Yamanaka, exploraron trabajosamente la combinacion de innumerables factores de transcripcion y llegaron a la conclusion de que la inclusion de tan solo tres de ellos --Gata4, Mef2c
y Tbx5-- eran suficientes para generar cardiomiocitos palpitantes a partir de fibroblastos de la piel.
, the transcription factor which regulates Bop gene in developing heart, is also known to be involved in hypertrophic response in the adult heart (6).
University of Texas (Austin, TX) has patented a new polypeptide and the gene encoding therefore, said gene being regulated in cardiac tissue by the transcription factor MEF2C
Lipton's laboratory, MEF2C
turns on specific genes which drive stem cells to become nerve cells.
is a transcription factor that turns on specific genes which then drive stem cells to become nerve cells.