qRT-PCR analysis confirmed that the mRNA levels of cyclin family members ( ccna1 , ccnb1 , ccnd1 , and cdk1 ), minichromosome maintenance proteins ( cdc45 , mcm2 , mcm3
, mcm4 , mcm5 , mcm6 , and mcm7 ), and proliferation cell nuclear antigen ( PCNA ) were significantly upregulated in ATF3-overexpressing CFs [Figure 3]a.
The PPI network consisting of induced genes (Figure 3(a)) contained a large cell cycle-related cluster with hubs and nodes related to S-phase (CCNB1 and CCNB2, CCND3, CDC16, MCM3
, MCM7, and CDC25) and chromosome separation (PLK1, ANAPC5, 7, CDC20, and TUBA1A).
DEGs in module up-1, such as POLR2G, POLR2F, DDX23, PRIM1, MCM3
, TK1, SNRNP40, SNRNP70, and RNASEH2A, were significantly enriched in three pathways of pyrimidine metabolism (P=1.15E-03), DNA replication (P=5.24E-03), and spliceosome (P=4.41E-02).
MCM3AP is an acetyltransferase that acetylases MCM3
, and plays a role in DNA replication (Takei et al.
Samples were collected at 6 m (MCM1 and MCM7) and 9 m (MCM2 and MCM8) in Lake Fryxell and 13 m (MCM3
and MCM5) and 18 m (MCM4 and MCM6) in Lake WLB to capture the microbiology of distinct vertical physicochemical and biogeochemical layers within the trophogenic zone (region where adequate light exists to support phytoplankton photosynthesis) of the two lakes.
 have shown by immunofluorescence that Xenopus MCM3
does not colocalize with sites of DNA replication.
They found two, MCM3
and MCM7, that limited HIF-1a's activity, and were also part of the DNA replication machinery.
We confirmed ER[alpha]-dependent responses to BPA, BPAF, and Zea by detecting endogenous gene expression of PR, GREBI (gene regulation by estrogen in breast cancer 1), MCM3
(minichromosome maintenance complex component 3), and SPUVE (also known as PRSS23; a member of the trypsin family of serine proteases) by real time-PCR (Figure 4).
The Brecon makes up part of the Third Mine Countermeasures Squadron (MCM3
) and carries a small detachment of Royal Marines.
cereus strain variations (MCM1 and MCM3
) found by Ferguson et al.
As shown in Figure 3A, the expression of pS2, MYC, GREB1, SPUVE, and MCM3
was potentiated by [E.sub.2] treatment; however, pretreatment with 5 mM MAA attenuated the estrogen-induced responses.
Two strains of Bacillus cereus (MCM1 and MCM3
) and one strain of B.