qRT-PCR forward and reverse primers for IL-6, MCL1, and MCL1S genes and for the housekeeping EF-2 gene (used for normalization) are listed in Table 1.
MCL1 expression can be induced by survival and differentiation signals such as cytokines and growth factors produced as a result of activation of a number of well-known signal transduction pathways (e.g., MAP kinases, PI3K/Akt, JAK/STAT, and NF[kappa]B) .
We showed for the first time that small inhibitory (si)RNA silencing of MCL1 in S.
Silencing of the MCL1 gene expression was accomplished by transfection of cells with specific siRNA or with negative control siRNA (Accell Smart pool and Control pool, resp., Thermo Scientific Dharmacon).
Among these genes, MCL1 plays a key role in macrophage survival [13,14].
aureus-induced cytoprotection with the expression of MCL1, we determined the time dependence of the induction of specific mRNA after macrophage challenge with bacteria.
Thus, to further determine whether the survival of infected macrophages in response to proapoptotic stimulants was dependent on Mcl-1 synthesis, RNA interference with siRNA was used to selectively silence MCL1 gene expression.
aureus followed by incubation for 6 h with a specific NF[kappa]B-inhibitor, and then the levels of MCL1 were assessed in comparison to untreated infected cells.
aureus are resistant to apoptosis (both induced and spontaneous), and this apparent cytoprotective effect is a consequence of the significant upregulation of antiapoptotic genes, especially those involved in the mitochondrial pathway, such as MCL1 .
In addition to enhanced MCL1 gene expression , this increase in Mcl-1 levels was due in large part to increased Mcl-1 stability.
Craig, "MCL1, a gene expressed in programmed myeloid cell differentiation, has sequence similarity to BCL2," Proceedings of the National Academy of Sciences of the United States of America, vol.
Craig, "MCL1 provides a window on the role of the BCL2 family in cell proliferation, differentiation and tumorigenesis," Leukemia, vol.