We repeated the genes which play an important role in the inflammatory response by qPCR, and the results showed that CBT not only downregulated the levels of NOD1/NF-[kappa]B, but also downregulated MAPK12
(Supplemental Figure 1); however, the effect on TLR4 was insignificant (Supplemental Figure 2).
The mammalian MAPK family consists of ERK, p38, and JNK with each member having several isoforms: ERK1 to ERK8; p38a (MAPK14), p38fi (MAPK11), p38g (MAPK12
), and p38S (MAPK13); and JNK1 (MAPK8), JNK2 (MAPK9), and JNK3 (MAPK10).
These genes are BDNF (brain-derived neurotrophic factor), NTRK2 (neurotrophic tyrosine kinase type 2 receptor, also known as TrkB), SH2B2 (SH2B adaptor protein 2, also known as rAPS), MAPK3 (mitogen-activated protein kinase 3, also known as Erk), MAPK12
(mitogen-activated protein kinase 12, also known as p38), MAPKAPK2 (mitogen-activated protein kinase-activated protein kinase 2), and ATF4 (activating transcription factor 4, also known as CREB).
In fact it appears that MAPK12
(p38[gamma]) arose from a tandem duplication of MAPK11 (p38[beta]) on chromosome 22 while MAPK14 (p38[alpha]) and MAPK13 (p38[delta]) subsequently resulted from a single segmental duplication of the MAPK11-MAPK12 gene unit on chromosome 6 .